Conference Papers

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Browsing Conference Papers by Author "Ekwumemgbo, Patricia Adamma"

Now showing 1 - 6 of 6
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    ISOLATION OF PROTHROMBIN FROM BOVINE PLASMA BY ION EXCHANGE CHROMATOGRAPHY AND GEL FILTRATION
    (2015) Ekwumemgbo, Patricia Adamma; Okotie, Omawumi Mercy
    Prothrombin among several plasma proteins is the most important in blood clotting process. Bovine blood was collected in sodium citrate, centrifuged to obtain plasma. The plasma was assayed for prothrombin using prothrombin time test assay method. The clotting time for the crude plasma was 56 seconds. The plasma was then adsorbed on barium sulphate and separated by anion exchange chromatography. 30 fractions were collected and assayed for prothrombin. Some fractions had complete coagulation within 45 - 50 minutes, while some fractions had partial coagulation within 60 - 65 minutes. These fractions were pooled together and further separated based on molecular weight by gel filtration. 20 fractions were collected and assayed for prothrombin. Some fractions had complete coagulation within the time range of 50 – 60 minutes, while some had partial coagulation within 65 - 70 minutes. From the result obtained prothrombin was successfully isolated from the bovine plasma
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    ISOLATION OF PROTHROMBIN FROM BOVINE PLASMA BY ION EXCHANGE CHROMATOGRAPHY AND GEL FILTRATION
    (2015) Ekwumemgbo, Patricia Adamma; Okotie, Omawumi Mercy
    Prothrombin among several plasma proteins is the most important in blood clotting process. Bovine blood was collected in sodium citrate, centrifuged to obtain plasma. The plasma was assayed for prothrombin using prothrombin time test assay method. The clotting time for the crude plasma was 56 seconds. The plasma was then adsorbed on barium sulphate and separated by anion exchange chromatography. 30 fractions were collected and assayed for prothrombin. Some fractions had complete coagulation within 45 - 50 minutes, while some fractions had partial coagulation within 60 - 65 minutes. These fractions were pooled together and further separated based on molecular weight by gel filtration. 20 fractions were collected and assayed for prothrombin. Some fractions had complete coagulation within the time range of 50 – 60 minutes, while some had partial coagulation within 65 - 70 minutes. From the result obtained prothrombin was successfully isolated from the bovine plasma.
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    LEAD CONCENTRATION IN PRIMARY SCHOOL SOIL-DUST IN NIGERIA, AFRICA
    (2013) Ekwumemgbo, Patricia Adamma; Omoniyi, Kehinde Israel
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    LIPID CHARACTERISTICS AND BIO-FUEL POTENTIALS OF SOME AFRICAN PLANT SEEDS
    (2011-11) Ekwumemgbo, Patricia Adamma; Eddy, Nnabuk Okon; Omoniyi, Kehinde Israel
    The study examines lipid characteristics and bio-fuel potentials of Jatropha curcus seeds oil (Fig. 1), Elaeis guineensis seeds oil (Fig. 2), Helianthus annus seeds oil (Fig. 3) and Glycine max seeds oil (Fig. 4). The seeds were collected from Nigeria in Africa cleaned, de-shelled, dried, ground, the lipids (oil) extracted and defatted in a soxhlet apparatus, using n-hexane. The extracted lipids were obtained by filtration and the hexane removed using rotary evaporator. The physicochemical (oil Content, acid value, % free fatty acids (FFAs), iodine value, saponification value, peroxide value, viscosity and density) analysis of lipids was carried out using official recommended methods while Fatty acid composition was determined using gas chromatography (GC)