ISOLATION OF PROTHROMBIN FROM BOVINE PLASMA BY ION EXCHANGE CHROMATOGRAPHY AND GEL FILTRATION
ISOLATION OF PROTHROMBIN FROM BOVINE PLASMA BY ION EXCHANGE CHROMATOGRAPHY AND GEL FILTRATION
No Thumbnail Available
Date
2015
Authors
Ekwumemgbo, Patricia Adamma
Okotie, Omawumi Mercy
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Prothrombin among several plasma proteins is the most important in blood clotting
process. Bovine blood was collected in sodium citrate, centrifuged to obtain plasma. The plasma
was assayed for prothrombin using prothrombin time test assay method. The clotting time for the
crude plasma was 56 seconds. The plasma was then adsorbed on barium sulphate and separated
by anion exchange chromatography. 30 fractions were collected and assayed for prothrombin.
Some fractions had complete coagulation within 45 - 50 minutes, while some fractions had
partial coagulation within 60 - 65 minutes. These fractions were pooled together and further
separated based on molecular weight by gel filtration. 20 fractions were collected and assayed
for prothrombin. Some fractions had complete coagulation within the time range of 50 – 60
minutes, while some had partial coagulation within 65 - 70 minutes. From the result obtained
prothrombin was successfully isolated from the bovine plasma.
Description
Conference proceeding
Keywords
Plasma,, prothrombin,, anion exchange chromatography,, gel filtration