VETERINARY MEDICINE

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Browsing VETERINARY MEDICINE by Subject "(JAKARI STRAIN),"

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    IN VITRO AND IN VIVO STUDIES ON CLOSTRIDIUM CHAUVOEI (JAKARI STRAIN) TOXIN
    (2006-09) MANASA, Yohana Sugun
    It has been documented that toxins from Clostridium chauvoei (Jakari strain) play a pathogenic role in blackleg. These toxins have not been characterized. It has therefore become necessary to characterize the toxins to enhance our understanding of the pathogenesis of blackleg. The production and properties of Clostridium chauvoei toxins was investigated in vitro and in vivo in mice given a minimum lethal dose of 0.2 ml intravenously (IV) after incubation at 370C anaerobically in toxin production medium for 24 and 48 hours. Clostridium chauvoei (Jakari strain) toxin was stable at 45 0C and 70 0C temperature for 5 minutes, while hemolytic activity was completely destroyed at 100 0C for 5 minutes. Chemical agents, like ether, and hydrochloric acid did not alter the hemolytic activity of the toxin. Phenol and methanol had partial effect and some reduction in hemolytic effect was observed at 624 and 128 end-points for phenol and methanol respectively. Formalin and sodium hydroxide rapidly and completely destroyed hemolytic activity of the toxins. Hemolytic actity of the toxin was stable at 4 0C of storage for two weeks. A decline was observed when the toxins were stored at 4 0C for more than two weeks.Gross and histopathologic lesions were observed in tissues of mice which died following intravenous administration of the toxins. Hemorrhages and congestion were observed in the internal organs (livers and lungs). Focal and diffused areas of necrosis and infiltration of mononuclear cells were lesions observed in kidney, liver and pancreas of inoculated mice.