IN VITRO AND IN VIVO STUDIES ON CLOSTRIDIUM CHAUVOEI (JAKARI STRAIN) TOXIN
IN VITRO AND IN VIVO STUDIES ON CLOSTRIDIUM CHAUVOEI (JAKARI STRAIN) TOXIN
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Date
2006-09
Authors
MANASA, Yohana Sugun
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Abstract
It has been documented that toxins from Clostridium chauvoei (Jakari strain) play a
pathogenic role in blackleg. These toxins have not been characterized. It has
therefore become necessary to characterize the toxins to enhance our understanding
of the pathogenesis of blackleg. The production and properties of Clostridium
chauvoei toxins was investigated in vitro and in vivo in mice given a minimum lethal
dose of 0.2 ml intravenously (IV) after incubation at 370C anaerobically in toxin
production medium for 24 and 48 hours. Clostridium chauvoei (Jakari strain) toxin
was stable at 45 0C and 70 0C temperature for 5 minutes, while hemolytic activity
was completely destroyed at 100 0C for 5 minutes. Chemical agents, like ether, and
hydrochloric acid did not alter the hemolytic activity of the toxin. Phenol and
methanol had partial effect and some reduction in hemolytic effect was observed at
624 and 128 end-points for phenol and methanol respectively. Formalin and sodium
hydroxide rapidly and completely destroyed hemolytic activity of the toxins.
Hemolytic actity of the toxin was stable at 4 0C of storage for two weeks. A decline
was observed when the toxins were stored at 4 0C for more than two weeks.Gross
and histopathologic lesions were observed in tissues of mice which died following
intravenous administration of the toxins. Hemorrhages and congestion were observed
in the internal organs (livers and lungs). Focal and diffused areas of necrosis and
infiltration of mononuclear cells were lesions observed in kidney, liver and pancreas
of inoculated mice.
Description
A thesis submitted to the Postgraduate School
Ahmadu Bello University In partial fulfillment of the requirments for
the Award of Master of Science
(M.Sc) in Veterinay Microbiology
Department of Veterinary Pathology and Microbiology,
Faculty of Veterinary Medicine,
Ahmadu Bello University,
Zaria, Nigeria
Keywords
VITRO,, VIVO STUDIES,, CLOSTRIDIUM CHAUVOEI,, (JAKARI STRAIN),, TOXIN.