MODULATORY ROLE OF RESVERATROL-INDUCED DIETARY RESTRICTION AND ENVIRONMENTAL ENRICHMENT ON NEUROBEHAVIOURAL OUTCOME IN YOUNG HEALTHY MICE
MODULATORY ROLE OF RESVERATROL-INDUCED DIETARY RESTRICTION AND ENVIRONMENTAL ENRICHMENT ON NEUROBEHAVIOURAL OUTCOME IN YOUNG HEALTHY MICE
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Date
2013-04
Authors
MUHAMMAD, Mustapha Sheh
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Abstract
Dietary Restriction (DR) otherwise known as Caloric Restriction (CR) has been generally
defined as consumption of nutritious diet that is 30% to 40% less in calories compared to
ad libitum diet while Environmental Enrichment (EE) is defined as a sustained and
progressive increase in cognitive and sensorimotor stimuli with aggregated voluntary
physical activity and complex social interactions. The aim of this experiment was to
investigate the modulatory role of Resveratrol induced CR and EE on neurobehavioural
responses in young healthy mice. Twenty five mice of both sexes were divided into five
groups of 5 animals each: group I served as the control and received
carboxymethylcellulose (CMC) 50 mg per kg/day orally, group II animals were maintained
on every other day feeding, group III animals received Resveratrol 50 mg/kg, suspended in
10 g/L of (CMC) orally per kg/day. Group IV animals received CMC and kept in an
Enriched Environment while group V animals received Resveratrol 50 mg/kg and kept in
an Enriched Environment. The treatment lasted for the period of four weeks. On day 26,
27 and 28 of the study period, the animals were subjected to neurobehavioural evaluation
of motor coordination, motor strength, learning and memory. Brain and plasma samples
were evaluated for lipid profile, lipid peroxidation and antioxidant enzymes. The results
showed significant increase (P < 0.05) in the transfer latency (acquisition) in the every
other day feeding group (69.20 ± 9.03 seconds) compared to the control group (36.80 ±
5.58 seconds). A significant increase (P <0.05) was observed in the concentration of low
density lipoprotein (LDL) in every other day feeding group (1.72 ± 0.12 mg/dl) and
environmental enrichment treatment group (1.73 ± 0.16 mg/dl) when compared to the
control group (1.11 ± 0.07 mg/dl). The results obtained also showed significant decrease < 0.05) in MDA concentration in the Resveratrol treatment group kept in EE (1.50 ± 0.05
IU/L) compared to the control (1.84 ± 0.09 IU/L) and GPx activity in the Resveratrol
treatment group kept in EE (41.00 ± 2.02 IU/L) compared to the control (59.00 ± 2.85
IU/L). In conclusion, the results obtained demonstrated that Resveratrol induced CR and
EE have no effects on neurobehavioural responses coupled with some significant
alterations in lipid profile, lipid peroxidation and antioxidant enzymes in young healthy
mice over a period of four weeks.
Description
A THESIS SUBMITTED TO THE
SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD
OF MASTER OF SCIENCE DEGREE IN
HUMAN PHYSIOLOGY
DEPARTMENT OF HUMAN PHYSIOLOGY
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
MODULATORY, ROLE, RESVERATROL, INDUCED, DIETARY, RESTRICTION, ENVIRONMENTAL, ENRICHMENT, NEUROBEHAVIOURAL, OUTCOME, YOUNG, HEALTHY, MICE