PHYTOCHEMICAL ANALYSIS AND MICROBIAL SCREENING OF EXTRACTS OF ERYTHRINA SENEGALENSIS .D.C
PHYTOCHEMICAL ANALYSIS AND MICROBIAL SCREENING OF EXTRACTS OF ERYTHRINA SENEGALENSIS .D.C
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Date
1996-11
Authors
DAN'AZUMI, ABDULRAHEEM SAL1HU
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Abstract
The extracts of root bark, stem bark and leaf of Erythrina senegalensis DC, a
shrub which grows in the Savannah regions of West Africa and traditionally used to treat
jaundice, lever, dysentery, gonorrhoea and in healing wounds, have been chemically
investigated and tested for microbial activity. Phytochemical analyses carried out on the
methanol extracts of the root bark, stem bark and leaf showed the presence of alkaloids,
saponins, steroidal nucleus, and tlavonoids. The extracts were active against Bacillus
sublilis, Candida albicans, Escherichia coli, and Staphylococcus aureus. Extraction of
fraction suspected to be rich in sapogenins using petroleum ether and ethylacetate was
carried out to determine the active components of the plant.
The ethylacetate extract was active in the Shrimp Lethality Bioassay, and yielded,
on purification, four isolates coded ESEASG 1, ESEASG 2, ESEASG 3, and
ESEASG 4 respectively. The four isolates inhibited the growth of Bacillus sublilis
while ESEASG 1 and 4 showed activity on Staphylococcus aureus in the antimicrobial
assay On the basis of IR and UV spectra and m.pt (uncorr) values. ESEASG 2 and -1
were suspected to be isomeric.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL,
AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL FULFILMENT
OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF
SCIENCE DEGREE IN ANALYTICAL CHEMISTRY
Department of Chemistry
Ahmadu Bello University,
Zaria. Nigeria.
November, 1996.
Keywords
PHYTOCHEMICAL,, ANALYSIS,, MICROBIAL,, SCREENING,, EXTRACTS,, ERYTHRINA SENEGALENSIS .D.C.