SEXUAL BEHAVIOUR OF FEMALE WISTAR RATS TREATED WITH HYDROMETHANOLIC FRUIT PULP EXTRACT OF Azanza garckeana F. Hoffm.

Simple item page
dc.contributor.authorAHMAD, AISHA
dc.date.accessioned2026-02-23T15:07:23Z
dc.date.available2026-02-23T15:07:23Z
dc.date.issued2023-07
dc.descriptionA THESIS DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTERS OF SCIENCE DEGREE IN HUMAN PHYSIOLOGY DEPARTMENT OF HUMAN PHYSIOLOGY, FACULTY OF BASIC MEDICAL SCIENCES, COLLEGE OF MEDICAL SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA
dc.description.abstractSexual dysfunction has been estimated to affect 40% of females worldwide. Ripened fruits of Azanza garckeana are consumed traditionally as an aphrodisiac without proper scientific investigation. This work was aimed at evaluating the sexual behavior of female Wistar rats treated with hydromethanolic fruit pulp extract of Azanza garckeana F. Hoffm. Azanza garckeana fruit was gotten from Kaltungo local government of Gombe state. The fruit pulp was air dried after removal of the seeds and pulverized. The pulverized sample was macerated in 70% methanol and 30% water for 72hrs, and then filtered. The filtrate was evaporated to dryness to obtain the extract. Twenty-five female Wistar rats and five (5) male Wistar rats were used for the study, the female animals were randomly divided into 5 groups (n= 5): Group I served as normal negative control and received 1ml/kg distilled water, group II served as positive control and received 100 μg estradiol valerate and 50 μg progesterone 48 hr and 5 hr respectively before sexual behavioural test, while group III, IV and V received 250 mg/kg, 500 mg/kg and 1000 mg/kg body weight of the Azanza garckeana extract respectively for 14 days orally before the sexual behavior test. Sexual behavioral tests were performed with sexually experienced male rats 1:1 after the treatment period in a unilevel pacing chamber and the process videotaped for proper scoring. Proceptive behaviors and Lordosis was extrapolated as index for the level of proceptivity and receptivity respectively in the female animals. At the end of the experiment, the rats were anaesthetized with combined doses of ketamine and diazepam injection (50mg/kg/10mg/kg i.p). Blood sample was collected via cardiac puncture for hormonal assays which were done using ELISA kit for estrogen, progesterone and testosterone. The ovaries and uterus were dissected out and fixed in 10% formalin for histological examination. The result showed a significant increase (P<0.05) in proceptivity and receptivity in the treatment (III, IV & V) and positive control (II) groups when compared with the negative control (I) group, indicating enhanced sexual behaviour. The hormonal assay results revealed significant (P<0.05) increase in the serum estrogen (P<0.05) and a significant decrease in serum progesterone in the extract treated groups except at 500 mg/kg dose where it was significantly increased (P<0.05) when compared to all groups. There was significant decrease in testosterone when compared to negative control in the extract treated groups and positive control. The time spent in the male chambers and the number of entries into the male chambers also significantly increased in the extract treated groups when compared to negative control. The histological sections of ovaries and uterus revealed normal uterine and ovarian histoarchitecture, however increased follicular development in the ovary and glandular hyperplasia in the uterus were seen in the treated groups when compared to negative control. In conclusion Azanza garckeana hydromethanolic fruit pulp extract demonstrated aphrodisiac property, and may be used for the treatment of women with hypoactive sexual desire.
dc.identifier.urihttps://kubanni.abu.edu.ng/123456789/13203
dc.language.isoen
dc.titleSEXUAL BEHAVIOUR OF FEMALE WISTAR RATS TREATED WITH HYDROMETHANOLIC FRUIT PULP EXTRACT OF Azanza garckeana F. Hoffm.
dc.typeThesis
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
SEXUAL BEHAVIOUR OF FEMALE WISTAR RATS TREATED WITH.pdf
Size:
909.54 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
College of Medical Sciences