IN VITRO ANTIOXIDANT AND BLOOD CLOTTING POTENTIALS OF METHANOL EXTRACTS OF FRESH LEAVES OF Ocimum basilicum, Ocimum gratissimum AND Ocimum suave

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dc.contributor.authorABDULWAHAB, RABIAT
dc.date.accessioned2017-01-31T09:21:55Z
dc.date.available2017-01-31T09:21:55Z
dc.date.issued2016-02
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILMENT FOR THE AWARD OF MASTERS OF SCIENCE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY ZARIAen_US
dc.description.abstractThe genus Ocimum is ranked high and considered one of the important genus of the lamiaceae family due to the extensive use of many of its specie in traditional medicine. The plants extracts are used traditionally for the treatment and management of several diseases such as infections of the skin, urinary and gastrointestinal tracts, nasal bleeding, headache, diarrhea, wart, worms, kidney function, anticonvulsant, cough, catarrh, cold, fever and chest pains. The DPPH method was used to evaluate the in vitro free radical scavenging activities of the plant extracts. Standard methods were used to study the phytochemical constituents, isolation and identification of active compounds as well as the study of the effect of the plants extracts on blood clotting. The preliminary phytochemistry revealed the presence of several phytochemicals such as flavonoids, tannins, saponins, steroids, anthraquinone, carbohydrate, cardiac glycosides and triterpenes. The quantitative phytochemistry showed that all the plants have flavonoids and saponin in abundance with methanol extract of O. basilicum registering the highest percentage of 88.28% and 72.38% respectively. The aqueous extract of O. basilicum registered the lowest percentage of flavonoid (54.84%) while the aqueous extract of O. suave had lowest saponin (13.47%). Tannins were less abundant in all the plant extracts. The Radical Scavenging Activities of the extracts revealed that the methanol fraction of O. basilicum has more activity when compared to other extracts as it had the lowest IC50 of 0.58mg/ml. The GC-MS analysis of the chromatographic fractions obtained from the fractionation of O. basilicum provided peaks of different bioactive compounds showing 9-Octadecenal, 1-fluorodecane and 4-methyl-1-decene as the major compounds constituting 37.93%, 11.96% and 10.88% respectively in fraction 1 while the major compounds identified in fraction 2 were Heptanoic acid 2-methyl-2butyl ester (32.21%), 9-Octadecenoic acid (Z)- (27.77%) and n-Hexadecanoic acid (19.10%). The effect of the plants extract on clotting time revealed that the methanolic extracts of O. basilicum and O. suave are potent blood coagulant as they had clotting time similar to the standards used for the study. The study has validated the traditional use of O. basilicum, O. gratissimum and O. suave in the treatment of several diseases. It also shows that the use of the plant extracts in the treatment and management of nasal bleeding and wound healing is accurate.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8599
dc.language.isoenen_US
dc.subjectVITRO ANTIOXIDANT,en_US
dc.subjectBLOOD CLOTTING POTENTIALS,en_US
dc.subjectMETHANOL EXTRACTS,en_US
dc.subjectFRESH LEAVES,en_US
dc.subjectOcimum basilicum,en_US
dc.subjectOcimum gratissimum,en_US
dc.subjectOcimum suave,en_US
dc.titleIN VITRO ANTIOXIDANT AND BLOOD CLOTTING POTENTIALS OF METHANOL EXTRACTS OF FRESH LEAVES OF Ocimum basilicum, Ocimum gratissimum AND Ocimum suaveen_US
dc.typeThesisen_US
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