U-V INACTIVATION OF BACILLUS SUBTILIS SPORES ON POLYETHYLENE •

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dc.contributor.authorIKPEME, SAMUEL EFFIONG
dc.date.accessioned2014-02-05T11:20:56Z
dc.date.available2014-02-05T11:20:56Z
dc.date.issued1990-10
dc.descriptionA Thesis submitted to the Post-Graduate School, Ahmadu Bello University, in partial fulfilment of the requirements for the degree of Master of Science (Biophysics)en_US
dc.description.abstractBacillus subtilis 168 spores were used to study the sterilising effect of far ultraviolet radiation on polyethylene surfaces. The sterilising effect of heat as well as a combination of both heat and far UV radiation was also studied. Wet and air-dried samples of wild-type B subtilis 168 spores, placed on aluminium-coated polyethylene surfaces, were exposed to both heat and 254nm UV radiation. After exposure, they were recovered as suspensions in water and assayed for colony forming ability. The results show that high fluences of 254nm UV radiation produced high inactivation, but also tailed survival curves. It remains an open question as to the cause of this tail. Air-dried samples on polyethylene were found to be more sensitive than wet samples for low UV fluences. However, at high fluences, there was practically no difference in the sensitivity of the two samples. There was evidence of some degree of synergism between heat and UV light in the inactivation process.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/318
dc.language.isoenen_US
dc.subjectU-Ven_US
dc.subjectINACTIVATIONen_US
dc.subjectBACILLUSen_US
dc.subjectSPORESen_US
dc.subjectPOLYETHYLENEen_US
dc.titleU-V INACTIVATION OF BACILLUS SUBTILIS SPORES ON POLYETHYLENE •en_US
dc.typeThesisen_US
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