U-V INACTIVATION OF BACILLUS SUBTILIS SPORES ON POLYETHYLENE •
U-V INACTIVATION OF BACILLUS SUBTILIS SPORES ON POLYETHYLENE •
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Date
1990-10
Authors
IKPEME, SAMUEL EFFIONG
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Abstract
Bacillus subtilis 168 spores were used to study the sterilising effect of far ultraviolet radiation on polyethylene surfaces. The sterilising effect of heat as
well as a combination of both heat and far UV radiation was also studied. Wet and air-dried samples of wild-type B subtilis 168 spores, placed on aluminium-coated
polyethylene surfaces, were exposed to both heat and 254nm UV radiation. After exposure, they were recovered as suspensions in water and assayed for colony forming ability. The results show that high fluences of 254nm UV
radiation produced high inactivation, but also tailed survival curves. It remains an open question as to the cause of this tail. Air-dried samples on polyethylene were
found to be more sensitive than wet samples for low UV fluences. However, at high fluences, there was practically no difference in the sensitivity of the two samples. There was evidence of some degree of synergism between heat and UV light in the inactivation process.
Description
A Thesis submitted to the Post-Graduate School, Ahmadu Bello University, in partial fulfilment of the requirements for the degree of Master of Science (Biophysics)
Keywords
U-V, INACTIVATION, BACILLUS, SPORES, POLYETHYLENE