PHYTOCHEMICAL ANALYSIS OF COCHLOSPERMUM TINCTORIUM A. RICH.
PHYTOCHEMICAL ANALYSIS OF COCHLOSPERMUM TINCTORIUM A. RICH.
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Date
1998-11
Authors
AUDU, MUSA AKPEMl
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Abstract
The extraction efficiency of continuous and batch extraction methods using four
solvent systems of increasing polarities namely petroleum spirit 40 - 60 °C, Ethyl ether,
80% acetone and methanol were studied on dried rhizome of Cochlospermum tinctorium A.
Rich.
Phytochemical screening of extracts obtained by both extraction methods revealed
the presence of alkaloids, tannins, saponins, cardiac glycosides, carbohydrates, flavonoids
and anthraquinone glycosides which are reported for the first time. Preparative TLC of the
ethyl acetate fraction with petroleum spirit (60 - 80 oC): ethyl acetate (3:2) yield extracts 1 -
6. Extracts 1 - 6 were characterized by RF value determination and reaction with spray
reagents. UV spectra analysis shows extracts 1, 2, 5 and 6 to be flavanone glycosides and 3
and 4 to be flavonol glycosides.
Cytotoxic test using Brine Shrimp Lethality assay was carried out on four crude
extracts. The LC50 value for n-butanol, 80% acetone and methanol extracts were 437 ± 8
ug/ml, 240 ± 3 ug/ml and 871+5 ug/ml respectively. Meanwhile the ethyl acetate extract
was lethal at all concentration level administered.
Antimicrobial sensitivity tests of ethyl acetate, n-butanol, 80% acetone and methanol
extract were carried out on Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella
pneumoniae and Esherichia coli. The zones of inhibition were measured .
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL AHMADU BELLO
UNIVERSITY IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE
AWARD OF THE DEGREE OF MASTER OF SCIENCE IN CHEMISTRY.
DEPARTMENT OF CHEMISTRY
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY,
ZARIA-NIGERIA
NOVEMBER, 1998.
Keywords
PHYTOCHEMICAL,, ANALYSIS,, COCHLOSPERMUM,, TINCTORIUM,, RICH.