PHYTOCHEMICAL AND MEDICINAL PROPERTIES OF EXTRACTS OF THE LEAVES OF PEUCEDANUM WINKLERI H. WOLFF
PHYTOCHEMICAL AND MEDICINAL PROPERTIES OF EXTRACTS OF THE LEAVES OF PEUCEDANUM WINKLERI H. WOLFF
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Date
2014-03
Authors
MADUMELU, MARK
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Abstract
Phytochemical investigation of the leaves of Peucedanum winkleri. H. Wolff, revealed the presence of carbohydrates, free reducing sugar, cardiac glycoside, saponins, steroids, flavanoids, alkaloids, tannins and triterpenes. The extracts were screened for their antimicrobial activity against Staphylococcus aureus, Methicillin resistant Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Corynebacterium ulcerans, Klebsiella pneumoniae, Streptococcus pyogenes, Candida albicans, Candida tropicalis, Candida krusei and Proteus mirabilis. The result indicated that the extracts inhibited the growth of one or more test pathogens. The ethyl acetate extract showed a broad spectrum of antimicrobial activity.4-(4-Chlorophenyl)-3-cyclohexyl-1-methyl-6-(2-naphthoyl)-1,2,3,4-tetrahydro-1,2,4,5-Tetrazine was isolated from the ethyl acetate extract of the plant leaves by means of Vacuum liquid chromatography (VLC), Thin layer chromatography (TLC) and Preparative thin layer chromatography (PTLC) using a solvent mixture of 1:1 v/v petroleum-ether and ethyl acetate. The stucture was established mainly by MS, IR, 1H and 13C NMR including DEPT spectroscopy. Also the isolated compound was able to inhibit the growths of some of the microorganisms used for the studies. The result of the study justified the use of the plant against several infectious diseases.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT FOR THE
AWARD OF A MASTER DEGREE IN ORGANIC CHEMISTRY
DEPARTMENT OF CHEMISTRY,
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY, ZARIA
NIGERIA
MARCH, 2014
Keywords
PHYTOCHEMICAL,, MEDICINAL,, PROPERTIES,, EXTRACTS,, LEAVES,, PEUCEDANUM WINKLERI, H. WOLFF.