PRODUCTION AND OPTIMIZATION OF GLUCOAMYLASE USING CANDIDA FAMATA ISOLATED FROM FERMENTED MASHED PINEAPPLE MUST UNDER SOLID STATE FERMENTATION

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dc.contributor.authorAKPAN, Grace Michael
dc.date.accessioned2018-09-27T09:19:03Z
dc.date.available2018-09-27T09:19:03Z
dc.date.issued2017-08
dc.descriptionA THESES SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA,NIGERIAen_US
dc.description.abstractThe present study was an investigation on comparative glucoamylase production by Candida famata isolated from spontaneously fermented pineapple must, using wheat bran, maize bran and the combination of both substrates in equal ratios under solid state fermentation conditions. Various cultural parameters were monitored and subsequently optimized for the production of glucoamylase using different agroindustrial residues and processing mills. The pretreatment of substrates was milling to 2 - 3mm particle size. Mixed bran of maize and wheat gave the highest glucoamylase yield of 25.34 U/ml under predetermined optimum fermentation conditions (30°C, pH 5.0, 106 cells/ml inoculum concentration, 1:3 substrate to nutrient solution ratio and 3 days of fermentation). In contrast, lower activity of 17.93 and 13.28 U/ml were obtained in wheat bran and maize bran respectively. The mixed bran served as a better substrate in the production of glucoamylase. Highlighting the potential of this approach as an alternative strategy for waste management and sustainable production of enzymes could perhaps act as a springboard for applicable source in many biotechnological processes.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10564
dc.language.isoenen_US
dc.subjectPRODUCTION,en_US
dc.subjectOPTIMIZATION,en_US
dc.subjectGLUCOAMYLASE,en_US
dc.subjectCANDIDA FAMATA,en_US
dc.subjectFERMENTED MASHED,en_US
dc.subjectPINEAPPLE,en_US
dc.subjectSOLID STATE FERMENTATION,en_US
dc.titlePRODUCTION AND OPTIMIZATION OF GLUCOAMYLASE USING CANDIDA FAMATA ISOLATED FROM FERMENTED MASHED PINEAPPLE MUST UNDER SOLID STATE FERMENTATIONen_US
dc.typeThesisen_US
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