PRODUCTION AND OPTIMIZATION OF GLUCOAMYLASE USING CANDIDA FAMATA ISOLATED FROM FERMENTED MASHED PINEAPPLE MUST UNDER SOLID STATE FERMENTATION

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Date
2017-08
Authors
AKPAN, Grace Michael
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Abstract
The present study was an investigation on comparative glucoamylase production by Candida famata isolated from spontaneously fermented pineapple must, using wheat bran, maize bran and the combination of both substrates in equal ratios under solid state fermentation conditions. Various cultural parameters were monitored and subsequently optimized for the production of glucoamylase using different agroindustrial residues and processing mills. The pretreatment of substrates was milling to 2 - 3mm particle size. Mixed bran of maize and wheat gave the highest glucoamylase yield of 25.34 U/ml under predetermined optimum fermentation conditions (30°C, pH 5.0, 106 cells/ml inoculum concentration, 1:3 substrate to nutrient solution ratio and 3 days of fermentation). In contrast, lower activity of 17.93 and 13.28 U/ml were obtained in wheat bran and maize bran respectively. The mixed bran served as a better substrate in the production of glucoamylase. Highlighting the potential of this approach as an alternative strategy for waste management and sustainable production of enzymes could perhaps act as a springboard for applicable source in many biotechnological processes.
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A THESES SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA,NIGERIA
Keywords
PRODUCTION,, OPTIMIZATION,, GLUCOAMYLASE,, CANDIDA FAMATA,, FERMENTED MASHED,, PINEAPPLE,, SOLID STATE FERMENTATION,
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