EFFECTS OF IRRADIATED ASPARAGINE ON YEAST CELLS

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dc.contributor.authorAkpa, Timothy Chidozie
dc.date.accessioned2014-02-13T09:23:00Z
dc.date.available2014-02-13T09:23:00Z
dc.date.issued1986-06
dc.descriptionA Thesis submitted to the postgraduate school Ahmadu Bello University, Zaria, in partial fulfillment of the requirements for the award of a Master or Science degree in Radiation Biophysics. Department of Physics Ahmadu Bello University, Zarla.en_US
dc.description.abstractHaploid and diplod cells of the bakers yeast, Saccharomyces cerevisiae, were grown in lOkGy and 50kGy irradiated asparagine treated Wickerham media, with an unirradiated asparagine medium as control. The method of cultivation was by inoculating about 2000 cells per mililiter from the same culture into a fresh medium and incubating for a day. This procedure was repeated daily for a period of at least eleven days,, thus bringing the the culmulative age of the cells up to 150 generations in semicontinuous logarithmic growth phase. The growth pattern, viability and mutation on the CAN gene were monitored. The CAN mutation in the diploid cells already carrying a 'CAN-deletion' was scored as recombination. Results showed no statistically significant difference between the various treatments and the control medium cells. It could be inferred, therefore, that irradiated asparagine iased as a sole source of nitrogen may not constitute a cytotoxic or genetic hazard to the cells. A possible, extension of this result-may be to -suggest that irradiation of nitrogen source in food may not make the food dangerous and may not constitute it into a genetic risk.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1339
dc.language.isoenen_US
dc.subjectEFFECTS,en_US
dc.subjectIRRADIATED,en_US
dc.subjectASPARAGINE,en_US
dc.subjectYEAST,en_US
dc.subjectCELLSen_US
dc.titleEFFECTS OF IRRADIATED ASPARAGINE ON YEAST CELLSen_US
dc.typeThesisen_US
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