EFFECTS OF IRRADIATED ASPARAGINE ON YEAST CELLS

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Date
1986-06
Authors
Akpa, Timothy Chidozie
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Abstract
Haploid and diplod cells of the bakers yeast, Saccharomyces cerevisiae, were grown in lOkGy and 50kGy irradiated asparagine treated Wickerham media, with an unirradiated asparagine medium as control. The method of cultivation was by inoculating about 2000 cells per mililiter from the same culture into a fresh medium and incubating for a day. This procedure was repeated daily for a period of at least eleven days,, thus bringing the the culmulative age of the cells up to 150 generations in semicontinuous logarithmic growth phase. The growth pattern, viability and mutation on the CAN gene were monitored. The CAN mutation in the diploid cells already carrying a 'CAN-deletion' was scored as recombination. Results showed no statistically significant difference between the various treatments and the control medium cells. It could be inferred, therefore, that irradiated asparagine iased as a sole source of nitrogen may not constitute a cytotoxic or genetic hazard to the cells. A possible, extension of this result-may be to -suggest that irradiation of nitrogen source in food may not make the food dangerous and may not constitute it into a genetic risk.
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A Thesis submitted to the postgraduate school Ahmadu Bello University, Zaria, in partial fulfillment of the requirements for the award of a Master or Science degree in Radiation Biophysics. Department of Physics Ahmadu Bello University, Zarla.
Keywords
EFFECTS,, IRRADIATED,, ASPARAGINE,, YEAST,, CELLS
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