EFFECTS OF IRRADIATED ASPARAGINE ON YEAST CELLS
EFFECTS OF IRRADIATED ASPARAGINE ON YEAST CELLS
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Date
1986-06
Authors
Akpa, Timothy Chidozie
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Abstract
Haploid and diplod cells of the bakers yeast,
Saccharomyces cerevisiae, were grown in lOkGy and
50kGy irradiated asparagine treated Wickerham media, with
an unirradiated asparagine medium as control. The method of
cultivation was by inoculating about 2000 cells per
mililiter from the same culture into a fresh medium and
incubating for a day. This procedure was repeated daily
for a period of at least eleven days,, thus bringing the
the culmulative age of the cells up to 150 generations in
semicontinuous logarithmic growth phase.
The growth pattern, viability and mutation on
the CAN gene were monitored. The CAN mutation in the
diploid cells already carrying a 'CAN-deletion' was
scored as recombination. Results showed no statistically
significant difference between the various treatments and
the control medium cells.
It could be inferred, therefore, that irradiated
asparagine iased as a sole source of nitrogen may not
constitute a cytotoxic or genetic hazard to the cells.
A possible, extension of this result-may be to -suggest
that irradiation of nitrogen source in food may not
make the food dangerous and may not constitute it into
a genetic risk.
Description
A Thesis submitted to the postgraduate school Ahmadu
Bello University, Zaria, in partial fulfillment of the
requirements for the award of a Master or Science degree
in Radiation Biophysics.
Department of Physics
Ahmadu Bello University, Zarla.
Keywords
EFFECTS,, IRRADIATED,, ASPARAGINE,, YEAST,, CELLS