EFFECT OF NAPHTHALENE ACETIC ACID (NAA) ON IN VITRO ROOTING OF REGENERATED MICROSHOOTS OF GROUNDNUT (ARACHIS HYPOGAEA L.)
EFFECT OF NAPHTHALENE ACETIC ACID (NAA) ON IN VITRO ROOTING OF REGENERATED MICROSHOOTS OF GROUNDNUT (ARACHIS HYPOGAEA L.)
No Thumbnail Available
Date
2012-12
Authors
Abdulmalik, M.M
Usman, I.S
Olarewaju, J.D.
Aba, D.A.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Successful utilization of tissue culture for the improvement and conservation of groundnut will be
made possible only with an efficient shoot proliferation, formation of well-developed root system in
microshoots, successful acclimatization and final establishment in field. A regeneration protocol
ensuring a high frequency rooting of microshoots derived from embryonic axes of four groundnut
genotypes was achieved using naphthalene acetic acid (NAA). Microshoots of 3cm length were
subcultured on Murashige and Skoog (MS) medium plus 30% sucrose, supplement with 1.0 or
1.5mg/L NAA and compared with a hormone free medium (control). Rooting was significantly
influenced by the presence and concentration of NAA. Microshoots subcultured in medium
supplemented with 1mg/L NAA rooted within 2weeks of subculture, producing the highest number
of roots/plantlet and root-induction frequency. However, increasing the concentration of the auxin
suppressed root length with the hormone free medium having the longest roots. Significant
genotypic effect was also observed, with Samnut 22 having more number of roots/plantlet and
root-induction frequency.
Description
postprint journal article
Keywords
EFFECT, NAPHTHALENE ACETIC ACID (NAA), groundnut,, VITRO ROOTING,