MOLECULAR CHARACTERISATION OF ROTAVIRUSES DETECTED IN CHILDREN UNDER THE AGE OF FIVE YEARS WITH DIARRHOEA IN KANO STATE-NIGERIA
MOLECULAR CHARACTERISATION OF ROTAVIRUSES DETECTED IN CHILDREN UNDER THE AGE OF FIVE YEARS WITH DIARRHOEA IN KANO STATE-NIGERIA
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Date
2011-11
Authors
AMINU, WADA-KURA
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Abstract
ABSTRACT
Rotaviruses are the most common cause of severe diarrhoeal illness in infants and young
children in both developing and developed countries resulting in much death in sub-Saharan
Africa and Nigeria. A total of 285 stool specimens were collected from November, 2009 to July,
2010.and analyzed for group A antigen using ELISA and RT-PCR, The prevalence of rotavirus
in this study was 36.5% (104/285). Murtala Muhammad Specialist Hospital had 44.4% while
Gwarzo General Hospital had the lowest prevalence rate of 21.4%. The virus was detected more
in males 37.2% (61/164) than in females 35.4% (43/121). However, there is no statistically
significant difference (χ2=0.11, P>0.05, df =1) observed between the prevalence in males and
females. Rotavirus was detected with the highest frequency among children in the age 41-60
months with 50% prevalence. The least prevalence was in age 31-40 months having 15.8%. The
prevalence was highest in April with 77.6% and lowest in July 12.2%. Pearson Chi square
analysis showed significant difference (χ2=27.720, P<0.05, df=1) of rotavirus infection in the
two different seasons. Three different Rotavirus VP4 P-genotypes (P[8], P[4], and P[6]) were
detected in this study. Mixed infection were detected and consist only of dual P-types of
P[8]+P[6]. Analysis of the Rotavirus strains indicates that P[6] was the most common Rotavirus
infection in the study population with frequency of 50 (48.5%). Six VP7 P-genotypes were
detected. The predominant genotype was G2 with a prevalence of 35.0%. The lowest was G9
which had a prevalence of 1.9%. The highest combination was found to be G2P[6] with 19.4%
frequency of occurrence. A single GNTP[8+6] (non-typeable G and P[8+6]) mixed combination
of infection was also found among the study population. Finally there is need for further studies
on the molecular characterization of rotavirus and the need to provide effective vaccine against
the viral infection cannot be over emphasized.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL,
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE
AWARD OF THE DEGREE OF MASTER OF SCIENCE (M.Sc) IN
MICROBIOLOGY
Keywords
MOLECULAR, CHARACTERISATION, ROTAVIRUSES, DETECTED, CHILDREN UNDER, DIARRHOEA