MOLECULAR CHARACTERISATION OF ROTAVIRUSES DETECTED IN CHILDREN UNDER THE AGE OF FIVE YEARS WITH DIARRHOEA IN KANO STATE-NIGERIA

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dc.contributor.authorAMINU, WADA-KURA
dc.date.accessioned2014-02-11T12:04:45Z
dc.date.available2014-02-11T12:04:45Z
dc.date.issued2011-11
dc.descriptionA THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE (M.Sc) IN MICROBIOLOGYen_US
dc.description.abstractABSTRACT Rotaviruses are the most common cause of severe diarrhoeal illness in infants and young children in both developing and developed countries resulting in much death in sub-Saharan Africa and Nigeria. A total of 285 stool specimens were collected from November, 2009 to July, 2010.and analyzed for group A antigen using ELISA and RT-PCR, The prevalence of rotavirus in this study was 36.5% (104/285). Murtala Muhammad Specialist Hospital had 44.4% while Gwarzo General Hospital had the lowest prevalence rate of 21.4%. The virus was detected more in males 37.2% (61/164) than in females 35.4% (43/121). However, there is no statistically significant difference (χ2=0.11, P>0.05, df =1) observed between the prevalence in males and females. Rotavirus was detected with the highest frequency among children in the age 41-60 months with 50% prevalence. The least prevalence was in age 31-40 months having 15.8%. The prevalence was highest in April with 77.6% and lowest in July 12.2%. Pearson Chi square analysis showed significant difference (χ2=27.720, P<0.05, df=1) of rotavirus infection in the two different seasons. Three different Rotavirus VP4 P-genotypes (P[8], P[4], and P[6]) were detected in this study. Mixed infection were detected and consist only of dual P-types of P[8]+P[6]. Analysis of the Rotavirus strains indicates that P[6] was the most common Rotavirus infection in the study population with frequency of 50 (48.5%). Six VP7 P-genotypes were detected. The predominant genotype was G2 with a prevalence of 35.0%. The lowest was G9 which had a prevalence of 1.9%. The highest combination was found to be G2P[6] with 19.4% frequency of occurrence. A single GNTP[8+6] (non-typeable G and P[8+6]) mixed combination of infection was also found among the study population. Finally there is need for further studies on the molecular characterization of rotavirus and the need to provide effective vaccine against the viral infection cannot be over emphasized.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1250
dc.language.isoenen_US
dc.subjectMOLECULARen_US
dc.subjectCHARACTERISATIONen_US
dc.subjectROTAVIRUSESen_US
dc.subjectDETECTEDen_US
dc.subjectCHILDREN UNDERen_US
dc.subjectDIARRHOEAen_US
dc.titleMOLECULAR CHARACTERISATION OF ROTAVIRUSES DETECTED IN CHILDREN UNDER THE AGE OF FIVE YEARS WITH DIARRHOEA IN KANO STATE-NIGERIAen_US
dc.typeThesisen_US
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