STUDIES ON GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND SIALIC ACIDS IN APPARENTLY HEALTHY AND HIV INFECTED INDIVIDUALS

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dc.contributor.authorABDULLAHI, Hadiza
dc.date.accessioned2015-03-27T08:49:22Z
dc.date.available2015-03-27T08:49:22Z
dc.date.issued2014-12
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY, FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA DECEMBER, 2014en_US
dc.description.abstractThe present study was carried out to determine the prevalence of Glucose-6-Phosphte Dehydrogenase (G-6-PD) deficiency in HIV infected individuals and determine its possible role in causing haemolytic anaemia in HIV infected individuals receiving treatment (on ARTs and prophylactics) and also determine Sialic Acid profile and Sialidase Activity in HIV infected and Apparently Healthy individuals, and also determine the relationship between the sialic acid levels and sialidase activity. Blood samples were collected from 200 subjects (150 HIV infected individuals and 50 apparently healthy individuals divided into four groups- HIV ART Naïve, HIV Stable, HIV-OI and Apparently Healthy). Complete Blood Count, Glucose-6-Phospahte Dehydrogenase (G-6-PD) activity, Erythrocyte Surface Sialic Acid (ESSA), Free Serum Sialic Acid (FSSA) concentrations and Sialidase activity were determined for all 200 subjects. Analysis of variance (ANOVA) was used to compare the results of the different groups of HIV infected individuals as well as controls. The mean haemoglobin (HGB), Packed Cell Volume (PCV) and Red Blood Cells (RBC) concentrations were significantly lower (P≤0.05) in the HIV groups compared with the apparently healthy groups. Anaemia and neutropaenia were the most common heamatological abnormalities observed in this study with highest prevalence of anaemia found in the ART naive group. A G-6-PD deficiency prevalence of 22.5 % was found among the study population with no significant difference between the groups, also, haemolytic anaemia was absent in all the G-6-PD deficient individuals. The mean FSSA was 0.4±0.4mg/ml. There was significant difference (p≤0.05) between some groups. The highest levels of FSSA was observed in the HIV ART naïve (0.65±0.5mg/ml). The mean ESSA value for the study population was 0.54±0.35mg/ml with no significant difference vii (p≤0.05) between groups. The mean sialidase activity values were 0.52±0.1 μmol/min/μl, 0.40±0.1 μmol/min/μl, 0.45±0.1 μmol/min/μl and 0.41±0.1 μmol/min/μl for the HIV ART naïve, HIV stable, HIV/OIs and apparently healthy groups respectively. No significant difference (p≤0.05) was found between groups and also in gender and age. The finding in this study of higher mean sialidase activity and FSSA levels in the ART naïve HIV group compared with other groups indicate that the virus and other opportunistic pathogens may be sialidase producers in vivo which cleave off sialic acids from erythrocytes surface, leading to high levels of FSSA, anaemia and neutropaenia seen in this group. The higher ESSA concentration found in the HIV stable group along with lowest FSSA concentration in the group suggests the presence of sialyltransferases.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6328
dc.language.isoenen_US
dc.subjectGLUCOSE-6-PHOSPHATE,en_US
dc.subjectDEHYDROGENASE,en_US
dc.subjectSIALIC ACIDS,en_US
dc.subjectAPPARENTLY HEALTHY,en_US
dc.subjectHIV,en_US
dc.subjectINDIVIDUALS.en_US
dc.titleSTUDIES ON GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND SIALIC ACIDS IN APPARENTLY HEALTHY AND HIV INFECTED INDIVIDUALSen_US
dc.typeThesisen_US
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