ISOLATION AND MOLECULAR CHARACTERIZATION OF VibriocholeraeFROM WATER SOURCES IN ZARIA, KADUNA STATE, NIGERIA

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dc.contributor.authorBULUS, GYAMNDAH HOPE
dc.date.accessioned2016-02-05T09:19:01Z
dc.date.available2016-02-05T09:19:01Z
dc.date.issued2015-01
dc.descriptionA THESIS SUBMITED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIAen_US
dc.description.abstractABSTRACT Water is life and has a profound effect on human health. Vibrio cholerae is autochthonous in aquatic environment and is an important human waterborne pathogen causing cholera.This study was designed to determine the presence of Vibrio cholerae in some water sources in Zaria, Nigeria. A total of 207 water samples from wells, boreholes, tap, street vendors, and rivers were collected from Samaru, Sabon Gari and Zaria city and filtered using the membrane filtration technique. The filtrate on the filter paper was plated on thiosulphate citrate bilesalt sucose agar(selective medium)with prior enrichment on alkaline peptone water. Presumptive isolates were identified and characterized using both conventional biochemical method and Microgen (MicrogenTM GnA+B–ID System) identification kit. Isolates were serotyped using Vibrio cholerae antisera kit (Deben Diagnostics, Ltd.) and confirmed using PCR to detect presence of rfbO1, rfbO139 and ompW (species specific) genes. Data were analyzed to determine percentage distribution and prevalence of the pathogen and Krsukal-Wallis test was employed to acertain the significance (P < 0.05). Out of nine (9) Vibrio species isolated, the conventional method identified two (2) isolates as V. cholerae while the PCR confirmed five (5) isolates. Vibro cholerae of the non O1/ non O139 serogroup were isolated from well, river and street vended water with a prevalence of 2.4% in Zaria. Overall, V. cholerae was predominant in river (33.3%) followed by well (4.9%) and least predominant in street vended water (2.7%). None was isolated from borehole and pipeborne water. Water samples from Sabon Gari were most contaminated with an occurrence of 3.2% followed by Samaru (2.6%) while the least contaminated water samples were from Zaria city (1.5%). Results of this study suggest that the use of PCR targeting ompW species specific gene of V.cholerae in viii combination with the conventional method would be an important tool in proper identification of V. cholerae. Isolation of V. cholerae from some of the water sources indicates that water plays a significant role in transmission of the organism, and drinking water from such contaminated sources could pose a threat to public health. Hence, government should ensure that potable water is made available and accessible to the populace.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7370
dc.language.isoenen_US
dc.subjectISOLATIONen_US
dc.subjectMOLECULARen_US
dc.subjectCHARACTERIZATIONen_US
dc.subjectVibriocholeraeen_US
dc.subjectWATER SOURCESen_US
dc.subjectKADUNAen_US
dc.titleISOLATION AND MOLECULAR CHARACTERIZATION OF VibriocholeraeFROM WATER SOURCES IN ZARIA, KADUNA STATE, NIGERIAen_US
dc.typeThesisen_US
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