MUTAGENIC AND RECOMBINOGMIC ACTION OF GAMMA - IRRADIATED GLUCOSE SOLUTIONS IN YEAST By

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Date
1986-04
Authors
ABDUL KADIRI, LAWAL
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Abstract
ABSTRACT The influence of irradiated glucose solutions on growth, mutation and recombination induction of the yeast, Saccharomyces cerevisige was investigated. Aqueous glucose solutions (10%) were irradiated at room temperatures, at dose rates of 19 gray(Gy) per minute, with 10 and 50 kilogray gammarays from a cobalt-60 source. The irradiated solutions ware incorporated into heat s t e r i l i z e d components of "the c e l l 's nutrient media. Two cell s t r a i n s , kept at exponential growth phase were chronically exposed to the media. No impairment to cell p r o l i feration was observed. Treated media did not influence the induction of mutation or recombination compared to the control media; the measured rates of mutations and recombinations are comparable to the spontaneous level. The results indicate the possible safety of radappertized foods with high sugar content at screening level.
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A Thesis submitted to the Postgraduate School, Ahmadu Bello University, Zaria, in p a r t i a l fulfilment of the requirements for the award of degree of Master of Science (Radiation Biophysics).
Keywords
MUTAGENIC AND RECOMBINOGMIC ACTION OF GAMMA - IRRADIATED GLUCOSE SOLUTIONS IN YEAST By, RECOMBINOGMIC ACTION, GAMMA, IRRADIATED GLUCOSE, SOLUTIONS IN YEAST, SOLUTIONS IN YEAST
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