CHEMOPREVENTIVE EFFECT OF GREWIA MOLLIS AND VITEX DONIANA LEAF SUPPLEMENTATION ON N-METHYL N-NITROSOUREA INDUCED COLON CANCER IN RATS

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dc.contributor.authorBAWALLA, IYABO BOLAJI
dc.date.accessioned2017-01-30T13:39:43Z
dc.date.available2017-01-30T13:39:43Z
dc.date.issued2015-03
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENT FOR THE AWARD OF MASTERS DEGREE IN BIOCHEMISTRYen_US
dc.description.abstractChemopreventive effect of G. mollis and V. doniana leaf supplementation on N-methylN-nitrosourea induced colon cancer in rats was investigated following supplementation in the diet at 0, 2.5, 5 and 10% levels. N-mehtyl-N-nitrosourea was administered intrarectally at a dosage of 1% three times a week for 10 weeks. The carcinoembyronic antigen assay (CEA) showed that the MNU induced colon cancer control fed basal diet had a significant (P < 0.05) increased CEA levels compared to MNU induced colon cancer treated groups of the leaves of both G. mollis and V. doniana supplemented diets except for 2.5% V. doniana leaf supplemented diet. However, there was no significant (P > 0.05) difference in the CEA levels of the different proportions of G. mollis leaf supplemented diets compared to the normal control. The histopathology of the colon of the MNU induced colon cancer treated group showed increase glandular excretion (more globlet cells) of the mucosa and improved epithelial architecture with increasing levels of supplementation of both leaf of G. mollis and V.doniana compared to the MNU induced colon cancer control with less glandular excretion (more globlet cells) and a distorted epithelial architecture. The histology of the liver and kidney of the MNU induced colon cancer group showed intense congestion of the intertubular spaces and sinusoidal capillaries with intense necrosis compared to the leaf treated groups with moderate necrosis and presence of eosinophilic materials in the lumen of the kidney tubules. There is a significant (P ˂ 0.05) decrease in feed intake of the MNU induced colon cancer control group compared to animals fed the normal control feed (control feed). All the MNU induced colon cancer rats given G. mollis and V. doniana leaf supplemented diets had no significant change (P ˂ 0.05) in the feed intake compared to the MNU induced colon cancer control. Evaluation of haematological parameter in rats with MNU induced colon cancer showed that there was no significant (P> 0.05) change in the white blood cell count, red blood cell count, platelet and the mean corpuscular volume of the MNU induced colon cancer basal control rats compared to normal control of both dietary supplementation of the leaf of G. mollis and V. doniana. Thiobarbituric acid reactive substances (TBARS) and superoxide dismutase (SOD) significantly (P < 0.05) increased in the liver and kidney of the untreated MNU induced control group while there was a significant (P < 0.05) reduction in the catalase (CAT) compared to the normal control. These results suggest that the leaf of G. mollis and V. doniana possess high cancer chemopreventive and antioxidant properties justifying their use in folklore medicineen_US
dc.identifier.urihttp://hdl.handle.net/123456789/8550
dc.language.isoenen_US
dc.subjectCHEMOPREVENTIVE EFFECT,en_US
dc.subjectGREWIA MOLLIS,en_US
dc.subjectVITEX DONIANA LEAF SUPPLEMENTATION,en_US
dc.subjectN-METHYL N-NITROSOUREA INDUCED,en_US
dc.subjectCOLON CANCER,en_US
dc.subjectRATS,en_US
dc.titleCHEMOPREVENTIVE EFFECT OF GREWIA MOLLIS AND VITEX DONIANA LEAF SUPPLEMENTATION ON N-METHYL N-NITROSOUREA INDUCED COLON CANCER IN RATSen_US
dc.typeThesisen_US
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