CHEMOPREVENTIVE EFFECT OF GREWIA MOLLIS AND VITEX DONIANA LEAF SUPPLEMENTATION ON N-METHYL N-NITROSOUREA INDUCED COLON CANCER IN RATS
CHEMOPREVENTIVE EFFECT OF GREWIA MOLLIS AND VITEX DONIANA LEAF SUPPLEMENTATION ON N-METHYL N-NITROSOUREA INDUCED COLON CANCER IN RATS
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Date
2015-03
Authors
BAWALLA, IYABO BOLAJI
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Abstract
Chemopreventive effect of G. mollis and V. doniana leaf supplementation on N-methylN-nitrosourea
induced colon cancer in rats was investigated following supplementation
in the diet at 0, 2.5, 5 and 10% levels. N-mehtyl-N-nitrosourea was administered
intrarectally at a dosage of 1% three times a week for 10 weeks. The carcinoembyronic
antigen assay (CEA) showed that the MNU induced colon cancer control fed basal diet
had a significant (P < 0.05) increased CEA levels compared to MNU induced colon
cancer treated groups of the leaves of both G. mollis and V. doniana supplemented diets
except for 2.5% V. doniana leaf supplemented diet. However, there was no significant
(P > 0.05) difference in the CEA levels of the different proportions of G. mollis leaf
supplemented diets compared to the normal control. The histopathology of the colon of
the MNU induced colon cancer treated group showed increase glandular excretion
(more globlet cells) of the mucosa and improved epithelial architecture with increasing
levels of supplementation of both leaf of G. mollis and V.doniana compared to the
MNU induced colon cancer control with less glandular excretion (more globlet cells)
and a distorted epithelial architecture. The histology of the liver and kidney of the
MNU induced colon cancer group showed intense congestion of the intertubular spaces
and sinusoidal capillaries with intense necrosis compared to the leaf treated groups with
moderate necrosis and presence of eosinophilic materials in the lumen of the kidney
tubules. There is a significant (P ˂ 0.05) decrease in feed intake of the MNU induced
colon cancer control group compared to animals fed the normal control feed (control
feed). All the MNU induced colon cancer rats given G. mollis and V. doniana leaf
supplemented diets had no significant change (P ˂ 0.05) in the feed intake compared to
the MNU induced colon cancer control. Evaluation of haematological parameter in rats
with MNU induced colon cancer showed that there was no significant (P> 0.05) change
in the white blood cell count, red blood cell count, platelet and the mean corpuscular
volume of the MNU induced colon cancer basal control rats compared to normal control
of both dietary supplementation of the leaf of G. mollis and V. doniana. Thiobarbituric
acid reactive substances (TBARS) and superoxide dismutase (SOD) significantly (P <
0.05) increased in the liver and kidney of the untreated MNU induced control group
while there was a significant (P < 0.05) reduction in the catalase (CAT) compared to the
normal control. These results suggest that the leaf of G. mollis and V. doniana possess
high cancer chemopreventive and antioxidant properties justifying their use in folklore medicine
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF
THE
REQUIREMENT FOR THE AWARD OF MASTERS DEGREE IN
BIOCHEMISTRY
Keywords
CHEMOPREVENTIVE EFFECT,, GREWIA MOLLIS,, VITEX DONIANA LEAF SUPPLEMENTATION,, N-METHYL N-NITROSOUREA INDUCED,, COLON CANCER,, RATS,