ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM CENTAUREA SENEGALENSIS [DC]
ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM CENTAUREA SENEGALENSIS [DC]
No Thumbnail Available
Date
2020-02
Authors
AHMED, Idris
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Centaurea senegalensis DC.is a plant commonly used in Nigeria traditional medicine for
treatment of stomach ache and pains. The aim is to isolate and characterize bioactive compound
from the aerial part of Centaurea senegalensis.The plant was extracted using maceration method
to give the crude methanol extract which was sequentially fractionated using hexane,
dichloromethane and ethyl acetate respectively. The crude methanol extract and solvent fractions
were subjected to phytochemical screeningand found to contain alkaloids, flavonoids, terpenes,
tannins, saponins and glycosides. Purification of the n-hexane (CSH) and dichloromethane
(CSD) fractionsusing silica gel column chromatography led to the isolation of taraxasteroland
eupatorinrespectively. The structural elucidations of the compounds were determined using FTIR,
1D and 2D-NMR.The crude methanol extract, fractions and the isolated compounds were
subjected to antimicrobial activity against selected gram-positive and gram-negative bacteria
(Shigella dysenteriae,Salmonella typhi,Escherichia coli,Staphylococcus aureus, Vancomycin
ResistantEnterococci, Methicillin ResistantStaphylococcus aureus) results indicated varying
growth inhibitions with zones of inhibition of 16-28 mm for crude methanol extract,13-31 mm
for solvent fractions and 10-15 mmfor isolated compounds. The minimum inhibitory
concentration (MIC) of the fractions was12.5 mg/ml on the Methicillin ResistantStaphylococcus
aureus andVancomycin ResistantEnterococci. However, the isolated compounds were found to
demonstrate activity on Methicillin resistance Staphylococcus aureus and Shigelladysenteriae
with 25 mg/ml as the MIC and 50 mg/mlas the MBC. In conclusion, the results of this study have
shown the importance of Centaurea senegalensis as a source of bioactive compounds with
potential for the treatment of bacterial infections.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILLMENT OF THE AWARD OF A MASTER DEGREE IN
ORGANIC CHEMISTRY
DEPARTMENT OF CHEMISTRY,
AHMADU BELLO UNIVERSITY,
ZARIA, NIGERIA
Keywords
ISOLATION,, CHARACTERIZATION,, BIOACTIVE COMPOUNDS,, CENTAUREA SENEGALENSIS.