ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM CENTAUREA SENEGALENSIS [DC]
ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM CENTAUREA SENEGALENSIS [DC]
| dc.contributor.author | AHMED, Idris | |
| dc.date.accessioned | 2021-11-04T07:49:37Z | |
| dc.date.available | 2021-11-04T07:49:37Z | |
| dc.date.issued | 2020-02 | |
| dc.description | A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE AWARD OF A MASTER DEGREE IN ORGANIC CHEMISTRY DEPARTMENT OF CHEMISTRY, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA | en_US |
| dc.description.abstract | Centaurea senegalensis DC.is a plant commonly used in Nigeria traditional medicine for treatment of stomach ache and pains. The aim is to isolate and characterize bioactive compound from the aerial part of Centaurea senegalensis.The plant was extracted using maceration method to give the crude methanol extract which was sequentially fractionated using hexane, dichloromethane and ethyl acetate respectively. The crude methanol extract and solvent fractions were subjected to phytochemical screeningand found to contain alkaloids, flavonoids, terpenes, tannins, saponins and glycosides. Purification of the n-hexane (CSH) and dichloromethane (CSD) fractionsusing silica gel column chromatography led to the isolation of taraxasteroland eupatorinrespectively. The structural elucidations of the compounds were determined using FTIR, 1D and 2D-NMR.The crude methanol extract, fractions and the isolated compounds were subjected to antimicrobial activity against selected gram-positive and gram-negative bacteria (Shigella dysenteriae,Salmonella typhi,Escherichia coli,Staphylococcus aureus, Vancomycin ResistantEnterococci, Methicillin ResistantStaphylococcus aureus) results indicated varying growth inhibitions with zones of inhibition of 16-28 mm for crude methanol extract,13-31 mm for solvent fractions and 10-15 mmfor isolated compounds. The minimum inhibitory concentration (MIC) of the fractions was12.5 mg/ml on the Methicillin ResistantStaphylococcus aureus andVancomycin ResistantEnterococci. However, the isolated compounds were found to demonstrate activity on Methicillin resistance Staphylococcus aureus and Shigelladysenteriae with 25 mg/ml as the MIC and 50 mg/mlas the MBC. In conclusion, the results of this study have shown the importance of Centaurea senegalensis as a source of bioactive compounds with potential for the treatment of bacterial infections. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/12692 | |
| dc.language.iso | en | en_US |
| dc.subject | ISOLATION, | en_US |
| dc.subject | CHARACTERIZATION, | en_US |
| dc.subject | BIOACTIVE COMPOUNDS, | en_US |
| dc.subject | CENTAUREA SENEGALENSIS. | en_US |
| dc.title | ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM CENTAUREA SENEGALENSIS [DC] | en_US |
| dc.type | Thesis | en_US |
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