INDUCTION OF MAMMARY TUMOUR IN ALBINO RATS BY 7,12-DIMETHYLBENZ(a) ANTHRACENE SUSPENDED IN TWO VEGETABLE OILS
INDUCTION OF MAMMARY TUMOUR IN ALBINO RATS BY 7,12-DIMETHYLBENZ(a) ANTHRACENE SUSPENDED IN TWO VEGETABLE OILS
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Date
1990-12
Authors
JAKES, OMAGE JEBBA
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Abstract
The susceptibility of female albino rats to mammary tumour induction
by 7, 12-di-methylbenz(a) anthracene (DMBA) dissolved in two types of oils
was investigated. Female albino rats (aged 54 days) were intubated
with BKBA (65mg/>;g body weight) dissolved in either sesame seed oil
(Group A) or soyabean oil (Group B). The Influence of DMBA on male rata
was alao investigated by intubating mole albino rate with DMBA in
soyabean oil (Group C) or sesame seed oil (Group D). The animals were fed
laboratory animal chow (Pfizer-Ladokun feed), weighed weekly and the
females were palpated for mammary tumour development starting 3 weeks
post intubation. At 42 weeks, all surviving animals were killed by
decapitation,. At necropsy, blood and tissue samples were taken for
biochemical analysis, haemntological studies and histopnthological
examination.
In the females, biochemical profile shows significantly (P < 0.05)
higher levels of total serum protein and total serum cholesterol in the
soyabean oil group than the sesame seed oil group. The liver weight was
also significantly (P < 0.05) higher in the soyabean oil group than the
sesame seed oil group. to significant (P > 0.05) differences were
observed between the two groups in growth rate, body weight gain,
haematocrit, haemoglobin and lymphocyte counts.
In the males, biochemical profile shows significantly (P < 0.05)
higher levels of total serum protein and total serum cholesterol in the
soyabean oil group than the sesame seed oil group. There was
significant (P < 0.05) difference in body weight gain and growth pattern
between the two treatment groups. There was significantly (P < 0.05> higher scrotal sac and liver weights in the sesame seed oil group than
the soyabean oil group. There was significant <P < 0.05) difference in
the haematocrit, and haemoglobin levels while no differences (P >
0.05) were observed in tne lymphocyte counts of both treatment groups.
While soyabean oil may be substituted for sesame seed oil as DMBA
solvent, the oil and the use of albino rats as a model for mammary
tumourigenes and scrotal sac development requires further
investigation.
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Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL, AHMADU BBLLO UNIVERSITY,
ZARIA, IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AVARD OF THE
DEGREE OF MASTER OF SCIENCE
Department of biochemistry
Faculty of Science
Ahmadu Bello University
Zaria
Keywords
INDUCTION,, MAMMARY TUMOUR,, ALBINO RATS,, 7,12-DIMETHYLBENZ,, ANTHRACENE SUSPENDED,, VEGETABLE OILS