IN VITRO ANTIMYCOBACTERIAL ACTIVITY OF FICUS SYCOMORUS ON SOME MYCOBACTERIUM TUBERCULOSIS STRAINS

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dc.contributor.authorMOHAMMED SONG, ABUBAKAR
dc.date.accessioned2018-09-03T09:22:40Z
dc.date.available2018-09-03T09:22:40Z
dc.date.issued2017-02
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF LIFE SCIENCES AHMADU BELLO UNIVERSITY ZARIAen_US
dc.description.abstractThe anti-mycobacterial activity of Ficus sycomorus (stem bark, root, leaves and fruits) was studied in vitro against standard Mycobacterium tuberculosis strains using standard Nitrate Reductase Assay techniques with isoniazid and rifampicin as controls. The results revealed that the n-hexane fruit extract had activity against all the tested Mycobacterium tuberculosis strains, the activity was observed as follows: 400, 200, and 100 μg/mL against susceptible strain, 400μg/mL against isoniazid resistant (R-INH) and rifampicin resistant (R-RIF) strains. The n-hexane leaves, stem bark and root extracts had no activity and also the methanol and aqueous extracts of fruit, stem bark, root, and leaves of Ficus sycomorus showed no activity against all the tested Mycobacterium tuberculosis strains. The n-hexane fruit extract were subjected to constitutive phytochemical analysis of which saponins and alkaloids had the highest percentage content of, (16.67±0.04 %) and (6.00±0.02 %) respectively. The n-hexane fruit extract was then partially fractionated using thin layer chromatography and Colum chromatography where four fractions A-D were obtained, of which B-D were more active than A. The minimum inhibitory concentration were determined from fraction B-D where fraction B had the lowest minimum inhibitory concentration against all the tested Mycobacterium tuberculosis strains. The minimum inhibitory concentrations were observed at 6.25μg/mL against susceptible strain and R-RIF, and 100μg/mL against R-INH. Therefore, these findings indicate that Ficus sycomorus could be used as herbal drug not only against Mycobacterium tuberculosis but also against Multi-Drug resistant tuberculosis.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10193
dc.language.isoenen_US
dc.subjectVITRO ANTIMYCOBACTERIAL ACTIVITY,en_US
dc.subjectFICUS SYCOMORUS,en_US
dc.subjectMYCOBACTERIUM TUBERCULOSIS STRAINSen_US
dc.titleIN VITRO ANTIMYCOBACTERIAL ACTIVITY OF FICUS SYCOMORUS ON SOME MYCOBACTERIUM TUBERCULOSIS STRAINSen_US
dc.typeThesisen_US
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