ISOLATION AND CHARACTERIZATION OF LOCAL STRAINS OF STREPTOMYCES FROM THE SOIL AND SCREENING FOR POSSIBLE ANTIBIOTIC ACTIVITY

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dc.contributor.authorOSOSANYA, ADEBIMPE D.
dc.date.accessioned2014-02-10T10:22:44Z
dc.date.available2014-02-10T10:22:44Z
dc.date.issued1994-06
dc.descriptionA THESIS SUBMITTED TO AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE IN INDUSTRIAL MICROBIOLOGYen_US
dc.description.abstractABSTRACT Fifteen soil samples obtained from the garden, refuse dump and grassland in Ahmadu Bello University, Zaria, were screened for Streptomyces spp. On the whole, 62 isolates of Streptomyces spp were obtained. Characterization of these isolates using the "ISP Criteria" gave 28 species of Streptomyces. 3.2% of the total isolates could not be characterized. Morphological and physiological characterization of the isolates based on the "ISP Criteria" showed that, the main coloration found in all the isolates were gray, yellow and white with the occurrence of 86.7%, 8.3% and 5.0%, respectively. The spore chain morphology was rectus-flexibilis (RF) for 22 isolates (36.7%) and spira (S) for 38 isolates (63.3%). All the isolates had smooth spore surfaces. Only 23.3% of the isolates were chromogenic. 76.7% of the isolates did not show any chromogenic reaction on any of the three test media. 70% of the isolates produced hydrogen sulphide, from either Peptone Iron Agar or Lead Acetate Agar. The carbon utilization test revealed that the isolates can utilize between 5-11 carbon sources. Based on the "ISP Criteria", 8 groups of organisms were obtained. The percentage of occurence of Group I to VIII are 6.4%, 24.2%, 16.1%, 37.0%, 1.6%, 4.8%, 1.6% and 4.8% respectively. Group IV had the highest percentage of occurrence. 32.3% of the total isolates were found to have antibiotic activity made up of 16.1% of isolates have antibacterial activity, 9.7% antifungal activity and 6.5% broad spectrum activity. vi.i.ien_US
dc.identifier.urihttp://hdl.handle.net/123456789/843
dc.language.isoenen_US
dc.subjectISOLATIONen_US
dc.subjectCHARACTERIZATIONen_US
dc.subjectSTRAINSen_US
dc.subjectSTREPTOMYCESen_US
dc.subjectSOILen_US
dc.subjectSCREENINGen_US
dc.subjectPOSSIBLEen_US
dc.subjectANTIBIOTIC ACTIVITYen_US
dc.titleISOLATION AND CHARACTERIZATION OF LOCAL STRAINS OF STREPTOMYCES FROM THE SOIL AND SCREENING FOR POSSIBLE ANTIBIOTIC ACTIVITYen_US
dc.typeThesisen_US
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