VALIDATION OF SUBMERGENCE TOLERANCE IN CANDIDATE ORYZA SPECIES WITH ART5 AND SC3 MARKERS

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dc.contributor.authorAZEEZ, Wasiu A.
dc.date.accessioned2017-11-16T09:10:59Z
dc.date.available2017-11-16T09:10:59Z
dc.date.issued2017-02
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTERS OF SCIENCE (M.Sc) DEGREE IN BIOLOGY. DEPARTMENT OF BIOLOGY, FACULTY OF LIFE SCIENCES AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThis research was conducted to validate the efficiency of ART5 and SC3 markers in identifying submergence tolerant genotypes and novel sources of tolerance to submergence between and within Oryza species.Twenty Nine (29) candidate genotypes which consisted of seventeen (17) Oryza sativa, three (3) lowland NERICAs and Nine (9) Oryza glabberimawere obtained from Africa Rice Center (Africa Rice), International Institute of Tropical Agriculture (IITA), Ibadan station, Nigeria. These genotypes included widely cultivated and released rice varieties, lines under trials and lines that served as donor parents to some abiotic stresses. They were phenotypically evaluated and genotypically validated following standard field and laboratory procedures. Rice seeds of each genotype were sown by adopting a completely randomized block design with three replications. Submergence treatment was administered by gradually increasing water level at 5cm per day until a maximum of 100cm water level was attained. Plants were submerged for a period of 14days. Nonsubmerged treatment served as control check. Genotypic validation with ART5 and SC3 markers was adopted. The amplified products were subjected to 2% agarose gel electrophoresis and 10% poly acrylamide gel electrophoresis (PAGE) for separation. The genetic associations among varieties were evaluated by comparing the amplicon of the genotypes against IR64 Sub1. Dendrograms were constructed to determine association with DARwin 6.0.01 software. Submergence tolerant rice genotypes (IR64 Sub1, FARO36, GAMBIAKA, and NERICA-L-52) showed high percentage survival (89%, 51%, 51% and 73.33% respectively) by exhibiting moderate plant height (23.67cm, 26.00cm, 24.33cm and 42.33cm respectively) and reduced number of leaves (3.33cm, 3.00cm, 3.33 and 6.33cm respectively). Six genotypes showed recovery at 7DAS (days after de-submergence). These recovery was lost at 14DAS thus validating the desubmergence evaluation protocol. FARO57, NERICA-L-52, IR64 Sub1, W 8 45 and FKR54 showed recovery ranging from 0-11.5% from 7 to 14DAS. Submergence significantly (P>0.01) induced plant mortality (57.67 – 100%). Upon genetic validation, IR64 Sub1 (tolerant check) clustered with moderately tolerant Oryza sativa genotypes but failed to cluster with NERICA-L-52 which displayed potentials for submergence tolerance. Conclusively, ART5 and SC3 submergence tolerance markers were species specific and not efficient in accurate validation of submergence tolerance between rice x species. This indicates the need for QTL analyses aimed at tagging submergence tolerant genes with large effect on other rice species.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/9416
dc.language.isoenen_US
dc.subjectVALIDATION,en_US
dc.subjectSUBMERGENCE TOLERANCE,en_US
dc.subjectCANDIDATE ORYZA SPECIES,en_US
dc.subjectART5 AND SC3 MARKERSen_US
dc.titleVALIDATION OF SUBMERGENCE TOLERANCE IN CANDIDATE ORYZA SPECIES WITH ART5 AND SC3 MARKERSen_US
dc.typeThesisen_US
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