VALIDATION OF SUBMERGENCE TOLERANCE IN CANDIDATE ORYZA SPECIES WITH ART5 AND SC3 MARKERS
VALIDATION OF SUBMERGENCE TOLERANCE IN CANDIDATE ORYZA SPECIES WITH ART5 AND SC3 MARKERS
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Date
2017-02
Authors
AZEEZ, Wasiu A.
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Abstract
This research was conducted to validate the efficiency of ART5 and SC3 markers in
identifying submergence tolerant genotypes and novel sources of tolerance to
submergence between and within Oryza species.Twenty Nine (29) candidate genotypes
which consisted of seventeen (17) Oryza sativa, three (3) lowland NERICAs and Nine
(9) Oryza glabberimawere obtained from Africa Rice Center (Africa Rice),
International Institute of Tropical Agriculture (IITA), Ibadan station, Nigeria. These
genotypes included widely cultivated and released rice varieties, lines under trials and
lines that served as donor parents to some abiotic stresses. They were phenotypically
evaluated and genotypically validated following standard field and laboratory
procedures. Rice seeds of each genotype were sown by adopting a completely
randomized block design with three replications. Submergence treatment was
administered by gradually increasing water level at 5cm per day until a maximum of
100cm water level was attained. Plants were submerged for a period of 14days. Nonsubmerged
treatment served as control check. Genotypic validation with ART5 and SC3
markers was adopted. The amplified products were subjected to 2% agarose gel
electrophoresis and 10% poly acrylamide gel electrophoresis (PAGE) for separation.
The genetic associations among varieties were evaluated by comparing the amplicon of
the genotypes against IR64 Sub1. Dendrograms were constructed to determine
association with DARwin 6.0.01 software. Submergence tolerant rice genotypes (IR64
Sub1, FARO36, GAMBIAKA, and NERICA-L-52) showed high percentage survival
(89%, 51%, 51% and 73.33% respectively) by exhibiting moderate plant height
(23.67cm, 26.00cm, 24.33cm and 42.33cm respectively) and reduced number of leaves
(3.33cm, 3.00cm, 3.33 and 6.33cm respectively). Six genotypes showed recovery at
7DAS (days after de-submergence). These recovery was lost at 14DAS thus validating
the desubmergence evaluation protocol. FARO57, NERICA-L-52, IR64 Sub1, W 8 45
and FKR54 showed recovery ranging from 0-11.5% from 7 to 14DAS. Submergence
significantly (P>0.01) induced plant mortality (57.67 – 100%). Upon genetic validation,
IR64 Sub1 (tolerant check) clustered with moderately tolerant Oryza sativa genotypes
but failed to cluster with NERICA-L-52 which displayed potentials for submergence
tolerance. Conclusively, ART5 and SC3 submergence tolerance markers were species
specific and not efficient in accurate validation of submergence tolerance between rice
x
species. This indicates the need for QTL analyses aimed at tagging submergence
tolerant genes with large effect on other rice species.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD
OF
MASTERS OF SCIENCE (M.Sc) DEGREE IN BIOLOGY.
DEPARTMENT OF BIOLOGY,
FACULTY OF LIFE SCIENCES
AHMADU BELLO UNIVERSITY,
ZARIA, NIGERIA
Keywords
VALIDATION,, SUBMERGENCE TOLERANCE,, CANDIDATE ORYZA SPECIES,, ART5 AND SC3 MARKERS