ISOLATION, CHARACTERISATION AND ANTIMICROBIAL ACTIVITY OF BIOACTIVE CONSTITUENTS FROM THE LEAF EXTRACT OF Adenodolichos paniculatus (Hua) Hutch. & Dalz (FABACEAE)

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dc.contributor.authorISYAKU, IBRAHIM
dc.date.accessioned2018-09-26T08:30:53Z
dc.date.available2018-09-26T08:30:53Z
dc.date.issued2017-10
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE IN ORGANIC CHEMISTRY DEPARTMENT OF CHEMISTRY, FACULTY OF PHYSICAL SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThe plant Adenodolichos paniculatus which is used in ethno - medicine for the treatment of blennorrhoea, diarrhea, dysentery, small pox, heart burn and wound dressing was investigated for its chemical constituents and antimicrobial activity. The leaves of this plant were subjected to microwave assisted extraction to obtain n – hexane, chloroform, ethyl acetate and methanol extracts. The extracts were then subjected to preliminary phytochemical screening which revealed the presence of alkaloids, anthraquinones, flavonoids, reducing sugars, saponins, tannins and steroids/terpenes. Purification through various column chromatography, the ethyl acetate extract led to the isolation of compounds X1 (mixture of stigmasterol and β – sitosterol) and X2 (nonanoic acid) after spectral analysis IR, 1H NMR and 13C NMR. Determination of inhibitory activity (sensitivity test) using agar well diffusion method showed activity i.e. minimum inhibitory concentration (MIC) against Staphylococcus aureus, Salmonella typhi, and Candida albicans ranging from 12 – 50 mg/ml, Minimum Bactericidal Concentration/ Minimum Fungicidal Concentration (MBC/MFC) ranging from 25 – 50 mg/ml, which is found to be bacteriostatic against Salmonella typhi. Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Bacillus subtili showed no MBC (Bacteriostatic) for the methanol, ethyl acetate, chloroform and n –hexane extract.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10554
dc.language.isoenen_US
dc.subjectISOLATION,en_US
dc.subjectCHARACTERISATION,en_US
dc.subjectANTIMICROBIAL ACTIVITY,en_US
dc.subjectBIOACTIVE CONSTITUENTSen_US
dc.subjectLEAF EXTRACT,en_US
dc.subjectAdenodolichos paniculatus (Hua)en_US
dc.subjectHutch,en_US
dc.subjectDalz (FABACEAE)en_US
dc.titleISOLATION, CHARACTERISATION AND ANTIMICROBIAL ACTIVITY OF BIOACTIVE CONSTITUENTS FROM THE LEAF EXTRACT OF Adenodolichos paniculatus (Hua) Hutch. & Dalz (FABACEAE)en_US
dc.typeThesisen_US
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