ISOLATION, CHARACTERISATION AND ANTIMICROBIAL ACTIVITY OF BIOACTIVE CONSTITUENTS FROM THE LEAF EXTRACT OF Adenodolichos paniculatus (Hua) Hutch. & Dalz (FABACEAE)
ISOLATION, CHARACTERISATION AND ANTIMICROBIAL ACTIVITY OF BIOACTIVE CONSTITUENTS FROM THE LEAF EXTRACT OF Adenodolichos paniculatus (Hua) Hutch. & Dalz (FABACEAE)
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Date
2017-10
Authors
ISYAKU, IBRAHIM
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Abstract
The plant Adenodolichos paniculatus which is used in ethno - medicine for the treatment
of blennorrhoea, diarrhea, dysentery, small pox, heart burn and wound dressing was
investigated for its chemical constituents and antimicrobial activity. The leaves of this
plant were subjected to microwave assisted extraction to obtain n – hexane, chloroform,
ethyl acetate and methanol extracts. The extracts were then subjected to preliminary
phytochemical screening which revealed the presence of alkaloids, anthraquinones,
flavonoids, reducing sugars, saponins, tannins and steroids/terpenes. Purification through
various column chromatography, the ethyl acetate extract led to the isolation of compounds
X1 (mixture of stigmasterol and β – sitosterol) and X2 (nonanoic acid) after spectral
analysis IR, 1H NMR and 13C NMR. Determination of inhibitory activity (sensitivity test)
using agar well diffusion method showed activity i.e. minimum inhibitory concentration
(MIC) against Staphylococcus aureus, Salmonella typhi, and Candida albicans ranging
from 12 – 50 mg/ml, Minimum Bactericidal Concentration/ Minimum Fungicidal
Concentration (MBC/MFC) ranging from 25 – 50 mg/ml, which is found to be
bacteriostatic against Salmonella typhi. Escherichia coli, Pseudomonas aeruginosa,
Salmonella typhi, Bacillus subtili showed no MBC (Bacteriostatic) for the methanol, ethyl
acetate, chloroform and n –hexane extract.
Description
A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE
STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE IN ORGANIC CHEMISTRY DEPARTMENT OF CHEMISTRY, FACULTY OF PHYSICAL SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
ISOLATION,, CHARACTERISATION,, ANTIMICROBIAL ACTIVITY,, BIOACTIVE CONSTITUENTS, LEAF EXTRACT,, Adenodolichos paniculatus (Hua), Hutch,, Dalz (FABACEAE)