DETERMINATION OF PHOSPHOROUS IN SOME BIOLOGICAL SYSTEMS USING KINETICS AND SPECTROPHOTOMETRY METHODS

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dc.contributor.authorJIBOKU, ABAYOMI OLUWATOSIN
dc.date.accessioned2014-02-11T09:36:47Z
dc.date.available2014-02-11T09:36:47Z
dc.date.issued1976-12
dc.descriptionA Thesis presented to AHMADU BELLO UNIVERSITY For the Degree of MASTER OF SCIENCE (ANALYTICAL CHEM.) AHMADU BELLO UNIVERSITY, Department of Chemistry, Zaria, NIGERIA.en_US
dc.description.abstractAnalysi»s for phosphorous in the seeds, back and pulpy part of five different species of pumpkin was carried out using the conventional specirophotonietrie method and the less common, recent rapid kinetics method, the basis of which is the fast reaction between phosphate and MO(Vl) in acid medium. 'The level of phosphate was determined in the various species and a number of useful indications revealed viz (i) phosphorous content appeared to vary with the pumpkin species being most in the species called curcubita moschata(ii) for a particular species of pumpkin, the phosphorous con-cent is most in the seeds and least in the pulpy parts. The rapid kinetics method of analysing phosphorous by converting phosphorous to phosphate using the 'digestion mixture' consisting of concentrated sulphuric acid, copper-sulphate, potassium hydrogen sulphate and little powdered selenium Jones, Lee, Peacocke (1951) and reacting with acid MO(Vl) in a stopped-flow spectrophotometer proved viable for the pulpy parts of the various species but failed for the seeds and the back. Analysis for phosphorous in the blood serum of two sets of guinea fowls fed separately on guinea corn and Livestock feed for a number of weeks was also attempted both spectrophotometrically and kinetically. The spectrophotometry and kinetics results for blood serum showed the expected variation of phosphorous in the two sets of fowls based on the phosphorous content of the commercial feeds. Also there seems tc be a somehow better agreement between the spectrophotometric and kinetics results for the blood serum than for the pumpkin species. The results for pumpkin species showed marked differences and possible reasons for discrepancies outlined in the body of the text (see chapter 5)en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1066
dc.language.isoenen_US
dc.subjectDETERMINATION,en_US
dc.subjectPHOSPHOROUS,en_US
dc.subjectBIOLOGICAL,en_US
dc.subjectSYSTEMS,en_US
dc.subjectUSING,en_US
dc.subjectKINETICS,en_US
dc.subjectSPECTROPHOTOMETRY,en_US
dc.subjectMETHODSen_US
dc.titleDETERMINATION OF PHOSPHOROUS IN SOME BIOLOGICAL SYSTEMS USING KINETICS AND SPECTROPHOTOMETRY METHODSen_US
dc.typeThesisen_US
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