DETERMINATION OF PHOSPHOROUS IN SOME BIOLOGICAL SYSTEMS USING KINETICS AND SPECTROPHOTOMETRY METHODS
DETERMINATION OF PHOSPHOROUS IN SOME BIOLOGICAL SYSTEMS USING KINETICS AND SPECTROPHOTOMETRY METHODS
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Date
1976-12
Authors
JIBOKU, ABAYOMI OLUWATOSIN
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Abstract
Analysi»s for phosphorous in the seeds, back and pulpy part of
five different species of pumpkin was carried out using the conventional
specirophotonietrie method and the less common, recent rapid
kinetics method, the basis of which is the fast reaction between
phosphate and MO(Vl) in acid medium. 'The level of phosphate was
determined in the various species and a number of useful indications
revealed viz (i) phosphorous content appeared to vary with the pumpkin
species being most in the species called curcubita moschata(ii) for a particular
species of pumpkin, the phosphorous con-cent is most in the seeds
and least in the pulpy parts.
The rapid kinetics method of analysing phosphorous by converting
phosphorous to phosphate using the 'digestion mixture' consisting of
concentrated sulphuric acid, copper-sulphate, potassium hydrogen
sulphate and little powdered selenium Jones, Lee, Peacocke (1951)
and reacting with acid MO(Vl) in a stopped-flow spectrophotometer
proved viable for the pulpy parts of the various species but failed
for the seeds and the back.
Analysis for phosphorous in the blood serum of two sets of
guinea fowls fed separately on guinea corn and Livestock feed for a
number of weeks was also attempted both spectrophotometrically and
kinetically.
The spectrophotometry and kinetics results for blood serum
showed the expected variation of phosphorous in the two sets of fowls
based on the phosphorous content of the commercial feeds. Also there
seems tc be a somehow better agreement between the spectrophotometric
and kinetics results for the blood serum than for the pumpkin species.
The results for pumpkin species showed marked differences and possible
reasons for discrepancies outlined in the body of the text (see chapter 5)
Description
A Thesis presented to
AHMADU BELLO UNIVERSITY
For the Degree of
MASTER OF SCIENCE (ANALYTICAL CHEM.)
AHMADU BELLO UNIVERSITY,
Department of Chemistry,
Zaria, NIGERIA.
Keywords
DETERMINATION,, PHOSPHOROUS,, BIOLOGICAL,, SYSTEMS,, USING,, KINETICS,, SPECTROPHOTOMETRY,, METHODS