MOLECULAR STUDY ON THE N GENE OF RABIES VIRUS ISOLATED IN DOGS FROM TWO SELECTED LOCAL GOVERNMENT AREAS OF BENUE STATE, NIGERIA
MOLECULAR STUDY ON THE N GENE OF RABIES VIRUS ISOLATED IN DOGS FROM TWO SELECTED LOCAL GOVERNMENT AREAS OF BENUE STATE, NIGERIA
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Date
2019-03
Authors
ADAJI, joseph otafu
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Abstract
Rabies is a viral zoonosis that causes encephalomyelitis. The rabies virus is a negative sense non-segmented RNA virus that codes for 5 proteins: N protein, P protein, M protein, G protein, and L protein. This research was aimed at carrying out molecular studies on a segment of N gene of rabies virus isolated from dogs in Benue State. The study revealed the presence of Nucleo protein antigen using the Direct Fluorescent Antibody Test (DFAT) in 2% of the 50 dog brain samples collected for the study. Sixty eight percent (68%) of the samples were from male dogs while 32% were from females. The 1 DFAT positive sample was from a male dog. The positive Sample (J50) with DFAT fluorescence score of (++++) was positive for Polymerase Chain Reaction (PCR) amplification of N-gene segment using N-gene specific forward primer; RabN-533F; 5‘-CATTGCAGATAGGATAGAGC-3‘ and reverse primer; RabN-888R; 5‘-GAGGAACGGCGGTCTCCTG-3‘ primer set. The amplified N gene segment of the isolate was sequenced and compared with 31 other rabies virus N gene sequences from the Gene bank. There was a maximum homology of 99% (KR080522 Dog from Bida 2012) and a minimum of 93% () . The group variation occurred at 32 different Non-serial positions of the nucleic acid of the gene, with a group variation rate of 8.3%. The phylogenic analysis gave an evolutionary pattern, showing the rabies virus is of West African origin belonging to the African 2 lineage and a possible history of Cameroonian origin considering that in an earlier year a similar rabies virus was isolated in that country and also might be associated with the fact that Benue state share border with the country.
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A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTERS DEGREE IN BIOTECHNOLOGY DEPARTMENT OF BIOCHEMISTRY, FACULTY OF LIFE SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
MOLECULAR STUDY,, GENE OF RABIES,, VIRUS ISOLATED IN DOGS,, LOCAL GOVERNMENT AREAS OF BENUE STATE,, NIGERIA,