ISOLATION AND CHARACTERIZATION OF FOUR BIOACTIVE TRITERPENOIDS FROM MUCUNA PRURIENS (DC) (FABACEAE) AND CANARIUM SCHWEINFURTHII (ENGL) (BURSERACEAE)

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dc.contributor.authorOKOLI, BAMIDELE JOSEPH
dc.date.accessioned2016-07-28T08:01:52Z
dc.date.available2016-07-28T08:01:52Z
dc.date.issued2015-11
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT FOR THE AWARD OF DOCTOR OF PHILOSOPHY IN ORGANIC CHEMISTRY Cover Page DEPARTMENT OF CHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractThe hexane, chloroform, ethylacetate and methanol extracts of C. schweinfurthii leaves showed high degree of ovicidal activity with 80 % inhibition at 10 μg/ml with chloroform crude extract being the most potent with a zone of inhibition of 24-26 mm against Gram-positive bacteria with a MIC of 12.5 mg/ml and MBC and MFC of 12.5 mg/ml. The chloroform and ethylacetate extracts of C. schweinfurthii stem bark showed high degree of larvicidal activity with 74 % inhibition at 10 μg/ml with ethylacetate crude extract showing a zone of inhibition of 22-33 mm against Gram-negative bacteria with a MIC of 12.5 mg/ml and, MBC and MFC of 25 mg/ml. The chloroform, ethylacetate and methanol extracts of M. pruriens showed potential ovicidal and larvicidal activities with 58 % inhibition at 10 μg/ml with chloroform crude extract having a zone of inhibition of 22-27 mm against Gram-negative bacteria with a MIC of 12.5 mg/ml and, MBC and MFC of 25 mg/ml. Four compounds were isolated – 3β-hydroxyllup-20(29)-en-28-oic acid (Betulinic acid) from the ethylacetate extract of C. schweinfurthii stem bark, a novel derivative of α-amyrin identified as 3β-hydroxyl olean-12,18-diene from the chloroform extract of C. schweinfurthii leaves, while stigmat-5-en-3β-ol (β-sitosterol) and naurol A were obtained from the chloroform extract of M. pruriens. 3β-hydroxyllup-20(29)-en-28-oic acid has the highest zone of inhibitions of 24 mm, 26 mm and 24 mm against Staphylococcus aureus, Bacillus subtlis and Candida albicans with MIC of 6.25 μg/ml against all tested organisms and MBC of 12.5 μg/ml and MFC of 25 μg/ml. 3β-hydroxyl olean-12,18-diene showed a comparative potency with zone of inhibitions of 29 mm, 32 mm and 26 mm against Methicillin Resistance Staphylococcus aureus , Bacillus subtlis and Candida albicans with MIC of 6.25 μg/ml and 12.5 μg/ml against the bacteria and candidas respectively, and MBC of 25 μg/ml and MFC of 25 μg/ml. The finding proved that 3β-hydroxyllup-20(29)-en-28-oic acid and 3β- hydroxyl olean-12,18-diene are the most potent isolated antimicrobial compounds. The most ovicidal compounds were 3β-hydroxyl olean-12,18-diene and stigmat-5-en-3β-ol with percentage inhibition of 62-65 % and 55-62 % respectively while the most larvicidal compounds viii were 3β-hydroxyllup-20(29)-en-28-oic acid ,3β-hydroxyl olean-12,18-diene and stigmat-5-en- 3β-ol at 78-83 %, 76-81% and 64–70 % at a concentration of 10-20 μg/ml respectively compared to albendazole.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8221
dc.language.isoenen_US
dc.subjectISOLATION,en_US
dc.subjectCHARACTERIZATION,en_US
dc.subjectFOUR BIOACTIVE TRITERPENOIDS,en_US
dc.subjectMUCUNA PRURIENS,en_US
dc.subject(DC),en_US
dc.subject(FABACEAE),en_US
dc.subjectCANARIUM SCHWEINFURTHII,en_US
dc.subject(ENGL),en_US
dc.subject(BURSERACEAE),en_US
dc.titleISOLATION AND CHARACTERIZATION OF FOUR BIOACTIVE TRITERPENOIDS FROM MUCUNA PRURIENS (DC) (FABACEAE) AND CANARIUM SCHWEINFURTHII (ENGL) (BURSERACEAE)en_US
dc.typeThesisen_US
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