ENHANCEMENT OF CELLULOSIC ETHANOL PRODUCTION THROUGH ASPERGILLUS NIGER MODIFICATION
ENHANCEMENT OF CELLULOSIC ETHANOL PRODUCTION THROUGH ASPERGILLUS NIGER MODIFICATION
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Date
2011-10
Authors
SHIRADIYI, JAMES MAIANGUWA
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Abstract
Simultaneous Saccharification and Fermentation (SSF) were carried out to produce ethanol from
maize stalk in 500ml conical flask. Aspergillus niger strains were isolated from four difference
sources, maize stalk, soil, bambaranut, rotten wood. Cellulose degrading ability was
screened by zone of clearance carried out by simple agar method.. Apergillus strain
from rotten wood (ANRW) produced the largest zone of clearance of 6.5mm; hence it was
selected for further studies. The effect of pH, temperature, substrate particle size, and substrate
concentration were studies and optimized to be 5.0, 3oC, 300um, 3% respectively.Aspergillus
niger was modified using UV irradiation technique by varying the exposure timings. The strain
expose at 30 minutes gave largest zone of clearance and hence was selected. The ethanol yield
by simultaneous saccharification and fermentation of modified and unmodified strain of A. niger
and saccharomyces cerevisae isolate from burkutu by Debo in Micro biology department ABU
Zaria was compared at optimum condition pH 5.0,temperature 30oC, 3%, substrate concentration
and 300um substrate particle respectively. The mutant strain from the UV irradiation gave the
maximum ethanol yieldof9.3g/100ml which is higher than that of parent strain 3.4g/100ml.
Description
A THESIS SUBMITTED CHEMICAL ENGINEERING DEPARTMENT, AHMADU BELLO
UNIVERSITY, ZARIA
Keywords
ENHANCEMENT,, CELLULOSIC,, ETHANOL,, PRODUCTION,, ASPERGILLUS,, NIGER,, MODIFICATION.