EVALUATION OF THE TRYPANOCIDAL ACTION OF COTTONSEED OIL ON Trypanosoma brucei AND Trypanosoma congolense, IN VITRO

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Date
1992-01
Authors
JEGA, MOHAMMED ABDULLAHI
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Abstract
The trypanocidal action of cottonseed oil was evaluated on T_. bcucei and T. congolense, in vitro. The organisms were maintained in MEM 199 and in coconut fluid medium for periods of up to 72 hours. MEM 199 was supplemented with L.cysteine (ImM), HEPES (25mM or lOmM), 2mM sod,ium pyruvate, 0.2mM 2-mercaptoethanol and 2ug/mL gentamycin. The coconut fluid medium, however, was supplemented only with 2% sodium pyruvate. In addition, horse and rabbit sera were also used in the supplementation of all the media. In all media, a temperature of 37°C and CO2 atmosphere were used. i Cotton seed oil with and without gossypol elicited trypanocidal activity. This suggests that other trypanocides in the oil, possibly sterculic and malvalic acids elicited trypanocidal activity in the oil containing no gossypol. The oil containing gossypol had greater trypanocidal action. Oil with no gossypol had ED50 values of 0.79% and 0.72% for T. brucei and 0.80% and 0.7 1% for T. congolense (in 1 and 3 hours, respectively), respectively. Standard gossypol and salicylhydroxamic acid (SHAM) were also shown to be trypanocidal, using the same protocol. ED50 values for gossypol were 82uM (T_. brucei) and 98uM (T_. congolense); the ED50 values for SHAM were 222uM (T. brucei) and 224uM (T. congolense). The test compounds in this study all exhibited trypanocidal activity on T. brucei and T. congolense. Trypanocidal effects of samples I and 2 may be due to the cyclopropene moiety of cyclopropenoid fatty acids and detergent like properties. Gossypol and SHAM elicit their trypanocidal activity via disruption of energy generation. Samples 1 and 2, thus, could add to the numerous potential trypanocides being investigated.
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A project report submitted to the Postgraduate School, Ahmadu Bello University Zaria in partial fulfillment of the requirements for the award of the degree of Master of Science in Biochemistry
Keywords
EVALUATION,, TRYPANOCIDAL,, ACTION,, COTTONSEED,, OIL,, Trypanosoma brucei,, Trypanosoma congolense,, VITRO
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