EVALUATION OF THE TRYPANOCIDAL ACTION OF COTTONSEED OIL ON Trypanosoma brucei AND Trypanosoma congolense, IN VITRO
EVALUATION OF THE TRYPANOCIDAL ACTION OF COTTONSEED OIL ON Trypanosoma brucei AND Trypanosoma congolense, IN VITRO
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Date
1992-01
Authors
JEGA, MOHAMMED ABDULLAHI
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Abstract
The trypanocidal action of cottonseed oil was evaluated on T_.
bcucei and T. congolense, in vitro. The organisms were maintained in
MEM 199 and in coconut fluid medium for periods of up to 72 hours.
MEM 199 was supplemented with L.cysteine (ImM), HEPES (25mM or lOmM),
2mM sod,ium pyruvate, 0.2mM 2-mercaptoethanol and 2ug/mL gentamycin.
The coconut fluid medium, however, was supplemented only with 2%
sodium pyruvate. In addition, horse and rabbit sera were also used
in the supplementation of all the media. In all media, a temperature
of 37°C and CO2 atmosphere were used.
i
Cotton seed oil with and without gossypol elicited trypanocidal
activity. This suggests that other trypanocides in the oil,
possibly sterculic and malvalic acids elicited trypanocidal activity
in the oil containing no gossypol. The oil containing gossypol had
greater trypanocidal action. Oil with no gossypol had ED50 values
of 0.79% and 0.72% for T. brucei and 0.80% and 0.7 1% for T. congolense
(in 1 and 3 hours, respectively), respectively. Standard gossypol
and salicylhydroxamic acid (SHAM) were also shown to be trypanocidal,
using the same protocol. ED50 values for gossypol were 82uM (T_.
brucei) and 98uM (T_. congolense); the ED50 values for SHAM were
222uM (T. brucei) and 224uM (T. congolense).
The test compounds in this study all exhibited trypanocidal
activity on T. brucei and T. congolense. Trypanocidal effects of
samples I and 2 may be due to the cyclopropene moiety of cyclopropenoid
fatty acids and detergent like properties. Gossypol and SHAM elicit
their trypanocidal activity via disruption of energy generation.
Samples 1 and 2, thus, could add to the numerous potential
trypanocides being investigated.
Description
A project report submitted to the
Postgraduate School, Ahmadu Bello University
Zaria in partial fulfillment of the
requirements for the award of
the degree of Master of Science in Biochemistry
Keywords
EVALUATION,, TRYPANOCIDAL,, ACTION,, COTTONSEED,, OIL,, Trypanosoma brucei,, Trypanosoma congolense,, VITRO