ANTISICKLING EFFECT AND ACTIVITY GUIDED FRACTIONATION OF DICHROSTACHYS CINEREA (Wight & Arn) ROOT AND STERCULIA SETIGERA (Del) LEAF METHANOLIC EXTRACTS ON HUMAN SICKLE RED BLOOD CELLS IN VITRO

Simple item page
dc.contributor.authorABDULLAHI, Baraka
dc.date.accessioned2018-08-15T08:29:53Z
dc.date.available2018-08-15T08:29:53Z
dc.date.issued2016-02
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThe in vitro antisickling effects of the crude methanolic extract, ethyl acetate, n-hexane, butanol, and aqueous fractions of methanolic extracts of Dichrostachys cinerea and Sterculia setigera were determined using different concentrates on sodium metabisulphite-induced sickled red blood cells. The result showed that antsickling activity was both concentration and time dependent, with highest activity observed at the longest time interval of 120 min, with the exception of n-hexane concentrate of 0.1mg/ml and aqueous fraction of 0.2mg/ml of Dichrostachys cinerea having a percentage unsickling effect of 38.46±1.4% and 33.39±1.4%, respectively. The ethylacetate butanol, n-hexane, and aqueous concentrates of Dicrostachys cinerea fractions showed a significant antisickling difference (p<0.05) when compared with p-hydroxybenzoic acid, (PABA) as positive control. However, the butanol concentrate of Sterculia setigera did not show any significant difference when compared with the positive control. Osmotic fragility test was carried out in saline concentrations of 0.1, 0.2, 0.3, 0.5, 0.6, 0.9ml using extract concentrations of 0.5, 1.0, 2.0, and 2.5mg/ml. A significant difference was observed in percentage inhibition of lysis in ethylacetate fraction of Sterculia setigera for all saline concentration when compared with a standard drug, Ibuprofen. For butanolic fraction of Sterculia setigera, there is no significant difference in percentage inhibition of haemolysis when compared with Ibuprofen in all tested saline concentrations. N-hexane fraction of Sterculia setigera showed a significant difference in percentage inhibition of haemolysis, when compared with Ibuprofen. Also, a significant difference was observed in percentage inhibition of lysis when compared with Ibuprofen. For ethylacetate fraction of Dicrostachys cinerea, a significant difference was observed in all tested saline concentrations, when compared with Ibuprofen. However, in the butanolic fraction, no significant difference in percentage inhibition of haemolysis was observed when compared to Ibuprofen. The n-hexane and aqueous fractions of Dicrostachys cinerea showed a significant difference in percentage lysis in all tested saline concentrations when compared with ibuprofen. The percentage methaemoglobin concentration was analysed using extract concentrations of 0.2, 0.4, 0.6, 0.8, and 1mg/ml. results showed that no significant difference was observed in all fractions of both test plants, when compared with the control. Elemental analysis was carried out using an AAS, the highest concentrations of Fe, Zn, Cu, and Cr were observed in aqueous fraction of Dichrostachys cinerea with a concentration of 287.20±0.00, 72.60±0.00, 57.20±0.00, 20.80±0.00 respectively, while the ethylacetate fraction has the least concentrations of the elements. Methanolic extracts of D. cinerea and S. setigera showed the highest antisickling activity. Aqeous fraction of S. setigera showed the highest methaemoglobin reduction property, while methanolic extract showed the best methaemoglobin reduction effect. Membrane protection activity was best in butanol fraction of D. cinerea, but methanolic extract of S. setigera had the highest membrane protection activity. The aqueous fraction of D. cinerea had the highest mineral concentration, while butanolic fraction of S. setigera had highest concentration of minerals. .en_US
dc.identifier.urihttps://kubanni.abu.edu.ng/handle/123456789/10109
dc.language.isoenen_US
dc.subjectANTISICKLING EFFECT,en_US
dc.subjectACTIVITY GUIDED FRACTIONATION,en_US
dc.subjectDICHROSTACHYS CINEREA,en_US
dc.subjectROOT,en_US
dc.subjectSTERCULIA SETIGERA,en_US
dc.subjectLEAF METHANOLIC EXTRACTS,en_US
dc.subjectHUMAN SICKLE RED BLOOD CELLS,en_US
dc.subjectVITROen_US
dc.titleANTISICKLING EFFECT AND ACTIVITY GUIDED FRACTIONATION OF DICHROSTACHYS CINEREA (Wight & Arn) ROOT AND STERCULIA SETIGERA (Del) LEAF METHANOLIC EXTRACTS ON HUMAN SICKLE RED BLOOD CELLS IN VITROen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ANTISICKLING EFFECT AND ACTIVITY GUIDED FRACTIONATION OF DICHROSTACHYS CINEREA.pdf
Size:
1.58 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
SCIENCE