MIDGUT EP PROTEIN GENE EXPRESSION IN GLOSSINA MORSITANS MORSITANS INFECTED WITH TRYPANOSOMA CONGOLENSE
MIDGUT EP PROTEIN GENE EXPRESSION IN GLOSSINA MORSITANS MORSITANS INFECTED WITH TRYPANOSOMA CONGOLENSE
| dc.contributor.author | GIMBA, Hassan Dongba, | |
| dc.date.accessioned | 2019-06-20T14:34:19Z | |
| dc.date.available | 2019-06-20T14:34:19Z | |
| dc.date.issued | 2018-11 | |
| dc.description | A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA NIGERIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN BIOTECHNOLOGY DEPARTMENT OF BIOCHEMISTRY FACULTY OF LIFE SCIENCES AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA | en_US |
| dc.description.abstract | Trypanosoma congolense, is a protozoan parasite that causes animal trypanosomosis also known as ‘Nagana’ in cattle. It is transmitted by the vector tsetse fly in which it undergoes several cyclical differentiation and development. The vector expresses a protein called Glutamic acid proline (EP) protein that plays an immunity role as an antagonist to the parasite establishment in the midgut. The present work was aimed to study the expression of the glutamic acid proline (EP) protein gene during the parasite establishment in the fly midgut at day 3, 10 and 24 post infections. One hundred teneral Glossina morsitans morsitans flies were fed on Trypanosoma congolense infected rat‟s blood, and only fifty four fully engorged flies were selected for the experiment. DNA was extracted from the anal dropping (s) of each of the fifty four flies for polymerase chain reaction to determined if the flies were infected. Twenty non-infected flies served as control. The flies were dissected and midguts collected at day 3, 10 and 24 post-infection. Reverse transcriptase Polymerase chain reaction (RT-PCR) followed by Quantitative polymerase chain reaction (qPCR) were performed on the midguts samples to determine the expression of Glutamic acid Proline (EP) protein. The results obtained, showed fly mortality of 29% (14/54), and percentage infection of 20% (8/40) by PCR. The qPCR result reveals that EP protein transcript was significantly (p< 0.05) more expressed at day 3 with fold change of 43.02 , as compared to day 10 and 24 having fold change of 1.22 and 1.27 respectively. Overall, the results have shown that the expression of EP protein gene in Glossina morsitans morsitansis a time dependent process, and could also be influenced by the species of the fly/trypanosomes interaction. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/11770 | |
| dc.language.iso | en | en_US |
| dc.subject | MIDGUT EP PROTEIN GENE EXPRESSION, | en_US |
| dc.subject | GLOSSINA MORSITANS MORSITANS INFECTED, | en_US |
| dc.subject | TRYPANOSOMA CONGOLENSE | en_US |
| dc.title | MIDGUT EP PROTEIN GENE EXPRESSION IN GLOSSINA MORSITANS MORSITANS INFECTED WITH TRYPANOSOMA CONGOLENSE | en_US |
| dc.type | Thesis | en_US |