PREVALENCE OF Staphylococccus aureus ISOLATED FROM BOVINE MASTITIS MILK IN ZARIA
PREVALENCE OF Staphylococccus aureus ISOLATED FROM BOVINE MASTITIS MILK IN ZARIA
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Date
2008-10
Authors
OKUNADE, EUNICE ADENIKE
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Abstract
This study investigates the prevalence of S. aureus isolated from bovine mastitic milk in
Zaria. The samples (n =350 quarters) were randomly collected from 91 lactating cows in
20 herds in Sabon-pegi, Shika, Sabon gari, Wusasa, Nuba, Bomo and Hanwa and 20% of
cows in each herds were sampled. The physicochemical quality, prevalence of subclinical
mastitis using California Mastitis Test (CMT) and bacteriological quality of the milk
samples were determined using standard methods. All the milk samples (100%) had no
clots, were colourless and consistent.The specific gravity and pH of the milk samples had
ranges of 1.023-1.033 and 3.11-6.99 respectively. The prevalence of subclinical mastitis
was 75 (21.4%). The prevalence of S. aureus and coagulase negative staphylococci
(CNS) in subclinical mastitis were 11 (14.7%) and 16 (21.3%) respectively, and in 350
quarter milk samples were 28 (8%) and 41 (11.7%) respectively. There was a statistical
significant difference (P < 0.05) in S. aureus isolated in the quarter milk samples that
were CMT positive than those that were CMT negative. There was also significant
association (P < 0.05) between the CMT positive milk samples and specific gravity, and
between S. aureus isolated and specific gravity of milk. The means ± standard deviation
of bacterial isolates of the bulk milk samples and their products ranged from 1.02±1.40
log10 cfu/ml - 2.05±1.05 log10cfu/ml and 1.13±1.77 log10cfu/ml – 1.40±1.62 log10cfu/ml
respectively and were within the acceptable microbial limit of <102 cfu/ml for S. aureus
and ≤ 5.0 x 102 cfu/ml for bacterial growth. Out of the 17 bulk milk sampled, 1 (5.9%) of
the bulk milk sample had antibiotic residues. S. aureus isolates in this study showed high
susceptibility (≥80% each) to all the antimicrobial agents [amoxicillin (25μg),
augumentin (30μg), chloramphenicol (30μg), cloxacillin (5μg), cotrimazole (25μg),
erythromycin (5μg), gentamycin (10μg) and tetracyclin (10μg)] used. The prevalence of
mastitis was high as to cause a substantial economic loss to farmers. In conclusion,
microbiological and epidemiological surveillance is necessary for effective treatment and
control of the disease. Hence, prevention and control of ssmastitis needs to be instituted.
Description
A DISSERTATION SUBMITTED TO THE POSTGRADUATE SCHOOL, AHMADU
BELLO UNIVERSITY, ZARIA NIGERIA
IN PARTIAL FULFILLMENT FOR THE AWARD OF
MASTER OF SCIENCE IN MICROBIOLOGY
DEPARTMENT OF MICROBIOLOGY
AHMADU BELLO UNIVERSITY,
ZARIA NIGERIA.
Keywords
PREVALENCE,, Staphylococccus,, aureus,, ISOLATED,, BOVINE,, MASTITIS,, MILK,, ZARIA