GASTROPROTECTIVE EFFECTS OF ZINC AND VITAMIN E ON SOME BIOCHEMICAL AND HISTOLOGICAL CHANGES ON ETHANOL-INDUCED MUCOSAL DAMAGE IN WISTAR RATS
GASTROPROTECTIVE EFFECTS OF ZINC AND VITAMIN E ON SOME BIOCHEMICAL AND HISTOLOGICAL CHANGES ON ETHANOL-INDUCED MUCOSAL DAMAGE IN WISTAR RATS
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Date
2014-06
Authors
OLAJIDE, Oladunni
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Abstract
Antiulcerogenic effects of zinc, vitamin E and their combination on ethanol-induced
ulcerogenic rats were investigated using standard methods. The rats treated with the
combination of zinc and vitamin E, followed by those treated with zinc alone and those
treated with the standard drug, cimetidine had significantly (P < 0.05) higher antiulcerogenic
properties as indicated by their preventive indices of 92.44 %, 89.92 %, and
73.11 % respectively compared to the rats treated with vitamin E alone (49.58 %).
Antioxidant studies revealed that vitamin E (10.01 ± 0.54 μmol/g tissue), zinc (8.77 ±
0.55μmol/g tissue) and their combination (8.52 ± 0.87 μmol/g tissue) exerted higher
protective effects against lipid peroxidation, which was significantly (P < 0.05) lower
than the ulcerogenic rats that were not treated and which had a value of 11.30 ±0.15
μmol/g tissue. Superoxide dismutase (2.08 ± 0.10 U/g tissue) and glutathione
peroxidase (43.17 ± 0.38 U/g tissue) activities increased significantly (P < 0.05) in the
ulcerogenic groups treated with vitamin E, compared with the normal control group
(1.63 ± 0.14 U/g tissue; 39.61 ± 3.18 U/g tissue). Catalase activity in the group treated
with vitamin E (49.61 ± 1.73 U/g tissue) showed no significant (P > 0.05) difference
compared with the normal control group (48.55 ± 2.76 U/g tissue) but was significantly
(P < 0.05) different from the group treated with zinc (42.64 ± 2.73 U/g tissue). There
was a significant (P < 0.05) increase in the packed cell volume (56.2 ± 3.01 %),
haemoglobin concentration ((17.62 ± 0.76 g/dl) and red blood cell count (10.22 ± 0.71 x
106/μl) of the ulcerogenic rats without treatment compared to all the other treatment
groups and the normal control group.The mean corpuscular volume of the treatment
groups indicated a significant (P < 0.05) increase (Vitamin E - 61.63 ± 0.22 fl; Zinc
alone - 60.97 ± 0.73 fl; and Zinc+ Vitamin E - 60.63 ± 1.53 fl.) as compared to the
normal control (55.07 ± 0.64 fl) and ulcerogenic rats without treatment (57.20 ± 0.96
fl). The leucocyte count decreased significantly (P < 0.05) in the treatment (Zinc - 7.2 ±
0.79 x 103/μl; Vitamin E - 5.8 ± 1.2 x 103/μl) and normal control groups (6.1 ± 1.3 x
103/μl) as compared to the ulcerogenic rats without any treatment (17.7 ± 1.7 x 103/μl).
The result of the study showed that zinc and/or vitamin E has antiulcerogenic properties
and that zinc and vitamin E enhance the integrity of blood cells in ulcerogenic rats. The
results showed that vitamin E and zinc supplements are of great value in the
development of new anti-ulcerogenic agents once the appropriate dosage and ratio are
utilized.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD
OF MASTERS DEGREE IN BIOCHEMISTRY.
DEPARTMENT OF BIOCHEMISTRY,
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY, ZARIA,
NIGERIA
JUNE, 2014
Keywords
GASTROPROTECTIVE,, ZINC,, VITAMIN,, BIOCHEMICAL,, HISTOLOGICAL,, ETHANOL,, MUCOSAL,, DAMAGE,, WISTAR RATS