STUDIES ON THE RESISTANCE OF HOST RED BLOOD CELLS TO TRYPANOSOMA VIVAX PHOSPHOLIPASES
STUDIES ON THE RESISTANCE OF HOST RED BLOOD CELLS TO TRYPANOSOMA VIVAX PHOSPHOLIPASES
No Thumbnail Available
Date
1988-10
Authors
OGBADOYI, EMMANUEL OLOFU
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Five goats were experimentally infected with Trypanesoma
vivax and the packed cell volume (PCV), haemoglobin (Hb)
levels and serum free fatty acid (FFA) levels were monitored.
A cyolical fluctuation was observed in the development of
parasitemia during the twenty-six-day period of infection
with the highest peaks on days five and twelve. There was
a general gradual decrease in both the PCV and the Hb levels
of all the infected animals, an indication of progressive
anaemia. The anaemia became very severe by day twenty-six
post infection as shown by the very low values of the PCV and
Hb levels. One of the infected animals died of acut infection
on day twenty-six post infection. Analysis for serum FFA
showed a general increase in the FFA levels of all the infected
animals throughout the period of infection.
Investigation into the haemolytic activity of the
isolated phospholipase A2 showed that the enzyme does not
cause any significant haemolysis of host (bovine) erythrocytes
in the absence of serum albumin. However, in the presence of
3.5% albumin, the enzyme caused up to 70% haemolysis. In the
presence of 20mM EDTA, a potent inhibitor of phospholipase
A2?, only 8.0% haemolysis was observed. The development of
drugs for the treatment of anaemia in trypanosomiasis using
inhibitors of phospholipases is therefore suggested
Description
A thesis submitted to the Post Graduate School, Department
of Biochmistry, Ahmadu Bello University, Zaria in partial
fulfilment of the requirements for the award of the Master of
Science Degree in Biochemistry.
Keywords
STUDIES, RESISTANCE, HOST, RED, BLOOD, CELLS, TRYPANOSOMA VIVAX, PHOSPHOLIPASES