DETECTION OF ANTIBIOTIC RESISTANCE AND VIRULENT GENES IN ENTEROCOCCI ISOLATED FROM WATER SAMPLES IN ZARIA, KADUNA STATE, NIGERIA.

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dc.contributor.authorKASHIM, Zainab Abimbola
dc.date.accessioned2017-08-02T14:41:18Z
dc.date.available2017-08-02T14:41:18Z
dc.date.issued2015-04
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER OF SCIENCE DEGREE (M.Sc.) IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractThe present study was conducted to isolate, identify and analyse the distribution of Enterococci,carry out antibiotic susceptibility test and molecularly detect the presence of some virulent and antibiotic resistantgenes in the Enterococci isolated from water samples in Zaria, Kaduna state. A total of two hundred and forty water samples were collected from different sources including wells, boreholes, tap and vended water (mairuwa). A total of34Enterococciwere isolated, characterized and identified. The prevalence ofEnterococcus avium and Enterococcus gallinarum in water were 58% and 24% respectively while Enterococcus mundtii and Enterococcus cecorum had 12% and 6%each. The mean frequency distribution was calculated using the one way ANOVA and based on this, the highest source of contamination was from wells, while vended water (mairuwa) had the least. Zaria city had the highest Enterococci isolates (44%) followed by Samaru (35%) and then Sabon gari (21%). Isolates had resistanceto the following antibiotics, Ampicillin 79% (10μg), Vancomycin 62%(30μg), Erythromycin 53%, (15μg), Chloramphenicol 12%, (10μg), Ciprofloxacin 12% (30μg), Tetracycline 12%, (12μg) and Gentamicin 3% (10μg). The result of the molecularscreening for van A,van B and cyl A genes showed the absence of all the genes in the isolates.The result of the present study indicatesthat the contamination of water with Enterococci could predispose to various health issues and pose serious therapeutic challenges due to the presence of resistance to antibiotics.The provision of proper water management systems and treated safe water supply to the communities cannot be overemphasized.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/9155
dc.language.isoenen_US
dc.subjectDETECTION,en_US
dc.subjectANTIBIOTIC RESISTANCE,en_US
dc.subjectVIRULENT GENES,en_US
dc.subjectENTEROCOCCI,en_US
dc.subjectWATER SAMPLESen_US
dc.subjectZARIA,
dc.subjectKADUNA STATE,
dc.subjectNIGERIA
dc.titleDETECTION OF ANTIBIOTIC RESISTANCE AND VIRULENT GENES IN ENTEROCOCCI ISOLATED FROM WATER SAMPLES IN ZARIA, KADUNA STATE, NIGERIA.en_US
dc.typeThesisen_US
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