IN-SILICO IDENTIFICATION OF POTENTIAL DRUG TARGETS FOR BOVINE ONCHOCERCIASIS USING PROTEINS FROM METABOLIC PATHWAYS OF Wolbachia ENDOSYMBIONT OF Onchocercaochengi

Simple item page
dc.contributor.authorOKPOKO, Cheluchi Solumtochukwu
dc.date.accessioned2021-11-04T07:47:49Z
dc.date.available2021-11-04T07:47:49Z
dc.date.issued2021-05
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY IN PARTIAL FULFILLMENT FOR THE AWARD OF MASTER DEGREE IN BIOTECHNOLOGY DEPARTMENT OF BIOCHEMISTRY, FACULTY OF LIFE SCIENCES AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractChemotherapy with antibiotics against the Wolbachia endosymbiont is a method of eliminating adult filarial parasites. Unfortunately, rapidly evolving drug resistance to antibiotics is limiting the use of these drugs against Gram-negative bacteria, thereby demanding novel approaches in drug target identification and chemotherapy. In-silico approaches offer a more rapid and cost effective strategy in drug discovery and design. In this study, computational techniques which involved a systematic protein subtractive approach, were employed to identify putative drug targets for bovine onchocerciasis, as a model of filariasis, using the biological pathways of Wolbachia endosymbiont of Onchocerca ochengi (wOo) available in KEGG (Kyoto Encyclopedia of Genes and Genomes). This study identified a total of 70 biological pathways of wOo from KEGG, with 13 of these pathways being unique to wOo and 57 being common to both cattle (Bos taurus) and wOo. Out of 349 proteins associated with the 70 pathways of Wolbachia of Onchocerca ochengi, 204 proteins were identified as non-homologous to cattle proteins by NCBI BLAST search tool. From the 204 proteins, 180 proteins were further identified as essential to the survival of Wolbachia of Onchocerca ochengi using DEG (Database of Essential genes). Prioritization of the resultant 180 proteins revealed 58 proteins as druggable targets for bovine onchocerciasis. Molecular docking studies of the three dimensional structures of these 58 proteins with 36 drug structures from drug bank database resulted in only 32 proteins of wOo completing the molecular docking process. This study therefore revealed 32 drug targets for bovine onchocerciasis. Molecular docking results of malic enzyme with NADH, N5-carboxyaminoimidazole ribonucleotide synthase with carglumic acid and replicative DNA helicase with zinc showed the best, moderate and least binding energies repectively. This study has identified potential drug targets of wOo for bovine onchocerciasis, towards control of human onchocerciasisen_US
dc.identifier.urihttp://hdl.handle.net/123456789/12691
dc.language.isoenen_US
dc.subjectIN-SILICO IDENTIFICATION,en_US
dc.subjectPOTENTIAL DRUG TARGETS,en_US
dc.subjectBOVINE ONCHOCERCIASIS,en_US
dc.subjectPROTEINS,en_US
dc.subjectMETABOLIC PATHWAYS,en_US
dc.subjectWolbachia ENDOSYMBIONT,en_US
dc.subjectOnchocercaochengi.en_US
dc.titleIN-SILICO IDENTIFICATION OF POTENTIAL DRUG TARGETS FOR BOVINE ONCHOCERCIASIS USING PROTEINS FROM METABOLIC PATHWAYS OF Wolbachia ENDOSYMBIONT OF Onchocercaochengien_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
IN-SILICO IDENTIFICATION OF POTENTIAL DRUG TARGETS FOR BOVINE.pdf
Size:
2.41 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
SCIENCE