SEROLOGICAL DIAGNOSIS OF HYDATIDOSIS IN ANIMALS SLAUGHTERED IN KANO ABATTOIR, KANO STATE, NIGERIA

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dc.contributor.authorLUKA, Sodangi Abdulkarim
dc.date.accessioned2021-06-17T08:24:31Z
dc.date.available2021-06-17T08:24:31Z
dc.date.issued2006-06
dc.descriptionA DISSERTATION SUBMITTED TO THE POSTGRADUATE SCHOOL AHMADU BELLO UNIVERSITY ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF DOCTOR OF PHILOSOPHY (Ph.D) IN VETERINARY PUBLIC HEALTH AND PREVENTIVE MEDICINE DEPARTMENT OF VETERINARY PUBLIC HEALTH AND PREVENTIVE MEDICINE, FACULTY OF VETERINARY MEDICINE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThe epidemiology of hydatidosis in animals including camels slaughtered in the Kano abattoir was investigated. Antigenic profiles and serum antibody responses from domestic animals naturally infected with Echinococcus granulosus were examined in Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting (WB) and Enzyme Linked Immunosorbent Assay (ELISA) using antigens prepared from E. granulosus hydatid cyst fluid. Available 10-year (1993-2002) hospital records on the disease in man were examined from four major hospitals in Kano municipal in order to determine the level of prevalence recorded in man. A total of 21 hydatid cysts were collected from goats (8), sheep (6) and camels (7). Five hundred and fifty one (551) serum samples were collected from sheep (138), goats(130), cattle(147) and camels(136) during the study period. The major antigenic components in the hydatid cyst fluid (HCF) antigen preparations were protein molecules of 12,45,50-66,118-160 kDa. Camel purified HCF showed prominent bands of 32,66,118,150 and 160 kDa.Goat purified antigen preparation showed prominent bands of 45-52,66,100 and118 kDa.The sheep HCF contained the 8,16,42,32-52,66,96,118 and 150 kDa bands respectively. The most consistent demonstrable protein in Echinococcus granulosus occurred as a complex in the 52-62 kDa region. Common antigenic bands occurred mainly between 30 and 67 kDa in electrophoresed cyst fluids of the animals. The overall prevalence of Echinococcus granulosus antibody response by ELISA was 32.6%. The highest prevalence was recorded in camels (38.9%), sheep (36.2%), cattle (31.2%) and goats had the least (23.8%). Higher prevalence was recorded in older animals than younger ones. There were no statistical significant differences between the prevalence rate of young and old cattle and camels (χ2=p>0.05), and no significant association between age and disease prevalence (p>0.05). Significant differences were recorded between young and adult sheep and goats (p<0.05). There were significant associations between age and prevalence in the sheep and goats (p<0.05). Antibodies to Echinococcus granulosus was encountered in both males and females with no statistical significant difference in prevalence between males and females among all the animals and no association between the sex of the animals and prevalence of antibodies to Echinococcus granulosus (p>0.05). Western blot analysis showed that 110 sera from all the animals representing 19.9% of the 551 serum samples bind at least one of the 8, 24, 30,36,38,42,48,52,66,92,118 or 150 kDa Echinococcus granulosus specific antigenic bands which served as criteria for determination of positivity. Bands of 66, 118 and 150 kDa persisted throughout the study among infected animals while the 8-24 kDa bands fluctuated. The abattoir was properly fenced, there were no stray dogs seen around the abattoir but the level of hygiene of the abattoir, method of disposal of animal wastes and condemnation of offals were very poor. Based on the hospital records, no egg (0.0%) or segment of Echinococcus was recovered in stool of patients that attended the four hospitals during the study period (ten year-record). Hospital surgery records showed that a hydatid cyst was recovered from the lung of a 30 year old male patient at the Mallam Aminu Kano Teaching Hospital in 1999. The study concludes that hydatidosis is prevalent in animals in the Kano area with potential health consequences in man.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/12452
dc.language.isoenen_US
dc.subjectSEROLOGICAL DIAGNOSIS,en_US
dc.subjectHYDATIDOSIS,en_US
dc.subjectANIMALS SLAUGHTERED,en_US
dc.subjectKANO ABATTOIR,en_US
dc.subjectKANO STATE,en_US
dc.subjectNIGERIA.en_US
dc.titleSEROLOGICAL DIAGNOSIS OF HYDATIDOSIS IN ANIMALS SLAUGHTERED IN KANO ABATTOIR, KANO STATE, NIGERIAen_US
dc.typeThesisen_US
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