ANTIBIOTIC RESISTANCE OF SOME GRAM NEGATIVE ORGANISMS FROM BEEF AND “TSIRE” SOLD IN AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.

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dc.contributor.authorHASSANA, RILWAN
dc.date.accessioned2017-05-08T09:04:35Z
dc.date.available2017-05-08T09:04:35Z
dc.date.issued2017-02
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThe use of antimicrobials in animal feed formulation has given rise to resistance noticedin species like Citrobacter spp, Enterobacter aerogenes,Escherichia coli, Klebsiella spp, Proteus spp, SalmonellasppandShigella spp, which are commonly associated with food borne diseases. This study was aimed at detection, characterization and determination of the antibiotic resistance of some Gram negative organisms from raw beef and ―Tsire‖ sold in Ahmadu Bello University Zaria, Nigeria. A total of 240 raw beef and processed beef (―Tsire‖) samples were collected and assayed for bacteria contaminants using standard cultural and biochemical methods. Susceptibility pattern of isolates to conventional antibiotics was also determined. Proximate composition of raw beef and ―Tsire‖ revealed the various proportions of moisture, protein, fats, carbohydrate and ash content. ―Tsire‖ had higher value than raw beef in all the parameters except in moisture. The total bacteria counts for the raw beef samples according to sales location were 2.61x104±4.89x103a (Faculty of Art), 4.57x104±8.3x103ab(Pepsi Garden), 3.57x104±6.89x103ab (Community Market) and 5.24x104±9.41x103b (Sculpture Garden) while the bacteria counts for the ―Tsire‖ samples according to sales location were 1.87x104±2.78x103a (Faculty of Art), 1.74x104±4.1x103a (Pepsi Garden), 1.37x104±1.77x103a (Community Market)and1.59x104±3.49x103a (Sculpture Garden). Statistical analysis of the mean bacterial load showed no significant difference (p>0.05). A total of 16 isolates were isolated namely E. coli (7 strains), Enterobacter sakazakii (3 strains), Hafnia alvei (4 strains), Klebsiella oxytoca and Tatumella ptyseos. All the isolates (100%) were susceptible to Imipenen, Nitrofuratoin and Chloramphenicol,81.25,69, 56, 50, 43.75, and 38% were susceptible to Gentamycin,Ampicillin, Tetracycline, Sulfamethoxazole/Trimethoprim, Amoxicillin Clavulanate, and Ciprofloxacine respectively. While31, 44,50, 37.5, 88% were resistant to Ampicillin, Tetracycline, Sulfamethoxazole/Trimethoprim, Amoxicillin Clavulanate and vii Erythromycin respectively. Polymerase Chain Reaction detection of tetA gene in Tetracycline resistant isolates revealed that only three (18.75%) of the Tetracycline resistant isolates possess TetA gene. The presence of these bacteria in meat should receive particular attention, because their presence indicates public health hazard and gives warning signals for the possible occurrence of food borne intoxication/infection.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8951
dc.language.isoenen_US
dc.subjectANTIBIOTIC RESISTANCE,en_US
dc.subjectGRAM NEGATIVE ORGANISMS,en_US
dc.subjectBEEF,en_US
dc.subject“TSIRE”en_US
dc.subjectAHMADU BELLO UNIVERSITY,en_US
dc.subjectZARIA,en_US
dc.subjectNIGERIAen_US
dc.titleANTIBIOTIC RESISTANCE OF SOME GRAM NEGATIVE ORGANISMS FROM BEEF AND “TSIRE” SOLD IN AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.en_US
dc.typeThesisen_US
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