ISOLATION, PARTIAL PURIFICATION, CHARACTERIZATION AND ANTIFUNGAL POTENTIAL OF CHITINASE FROM SEEDS OF SPHENOSTYLIS STENOCARPA (AFRICAN YAM BEANS)

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dc.contributor.authorOBIAKOR, Chimee Ethel
dc.date.accessioned2017-12-21T12:58:24Z
dc.date.available2017-12-21T12:58:24Z
dc.date.issued2017-03
dc.descriptionTHESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER DEGREE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractThe utilization of chemical fungicide has attracted increased scrutiny since they cause environmental contamination and induce pathogen resistance. These limitations calls for harmless alternative control strategy to prevent plant diseases for sustainable agriculture. This work was designed to partially purify,characterize and tested for the antifungal potentials of chitinase from seeds of African Yam Beans. Chitinase (EC.3.2.14) was isolated from Stephynostylis stenocarpa, by precipitation using 80% Ammonium Sulphate Saturation and Gel filtration on Sephadex G-75 column to 8.04 fold with a yield of 46.35% and a final specific activity of 4.45μmol/min. SDS-PAGE of the enzyme showed a molecular weight of 32KDa. The enzyme had an optimal temperature and pH of 55oC and 5.0 respectively. It had temperature and pH stability within the range of 30-60oC and 4-7 respectively. Initial velocity studies for the determination of kinetic constants with colloidal chitin as substrate revealed a KM and Vmax of 1.2mMand 12.29μmol/min. The Chitinase activity was inhibited by Pb2+, Hg2+, K+ and other reagents like EDTA and glucose but Na+ and Zn2+ showed little or no effect on the enzyme activity. The antifungal potentials of this Chitinase was tested against Fusarium oxysponium, Fusarium solani, and Alterneria alternate. It had no effect on the Fusarium species but inhibited the growth of Alterneria alternate as seen from clear Zones of inhibition (14.00mm-17.00mm) for the crude extract and (9.00mm-11.00mm) for the purified using agar diffusion method. This indicates that the enzyme can be used to combat such diseases triggered by the organism e.g. leaf blight, hence servingas a tool for crop protection in the agricultural sectoren_US
dc.identifier.urihttp://hdl.handle.net/123456789/9873
dc.language.isoenen_US
dc.subjectSOLATION,en_US
dc.subjectPARTIAL PURIFICATION,en_US
dc.subjectCHARACTERIZATION,en_US
dc.subjectANTIFUNGAL POTENTIAL,en_US
dc.subjectCHITINASE,en_US
dc.subjectSEEDS,en_US
dc.subjectSPHENOSTYLIS STENOCARPA,en_US
dc.subject(AFRICAN YAM BEANS),en_US
dc.titleISOLATION, PARTIAL PURIFICATION, CHARACTERIZATION AND ANTIFUNGAL POTENTIAL OF CHITINASE FROM SEEDS OF SPHENOSTYLIS STENOCARPA (AFRICAN YAM BEANS)en_US
dc.typeThesisen_US
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