SEROLOGICAL AND PCR DETECTION OF CYTOMEGALO VIRUS INFECTION AMONG PREGNANT WOMEN ATTENDING SOME HOSPITALS IN LOKOJA KOGI STATE, NIGERIA
SEROLOGICAL AND PCR DETECTION OF CYTOMEGALO VIRUS INFECTION AMONG PREGNANT WOMEN ATTENDING SOME HOSPITALS IN LOKOJA KOGI STATE, NIGERIA
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Date
2022-11
Authors
BALA, HANNATU ELEOJO MARY
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Abstract
Cytomegalovirus (CMV) is one of the most common virusesthat causes congenital defects
in babies from infected mothers. Most cases of congenital defects are asymptomatic at birth
but can lead to sensoneural hearing loss (SNHL) and neurodevelopmental delay which can
appear later in childhood. This study was conducted to determine the cytomegalovirus
infection among pregnant women attending antenatal clinics at Kogi State Specialist
Hospital (KSSH) and Federal Medical Centre (FMC) Lokoja, Kogi state, Nigeria. A total
of 184 serum samples were collected and analyzed using CMV IgM ELISA kit Diagnostic
Automation, INC (USA).The study revealed that 40 out of the 184 samples turned out
positive for CMV IgM antibodies giving a prevalence of 21.7% while the negative sample
result had a prevalence of 78.3%.From the forty (40) positive samples, seven (7) samples
were subjected to Polymerase Chain Reaction but it turned out negative. Although PCR is a
very sensitive and reliable method for detecting the organism (CMV) it was discovered that
peripheral blood leukocytes is a better medium of choice to use as against plasma which
was used for the research. Based on the research, there was no significant association
between socio-demographic factors such as marital status, type of marriage, occupation and
scarification and the infection. This could be due to the limited number in sample size.
Other socio economic factors such as education had a significant p value of 0.035 with the
infection. In addition, the pregnant women who had deformed babies, do not practice hand
wash and were not transfused had a significant difference with the infection (p= 0.046,
0.027 and 0.003 respectively).It is advised that screening and sensitization of CMV for
pregnant women when they come for ante-natal classes be done in order to reduce the risk
of congenital birth defects among infants.
Description
A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE
STUDIES, AHMADU BELLO UNIVERSITY IN PARTIAL FULFILLMENT OF
THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE
IN MICROBIOLOGY
DEPARTMENT OF MICROBIOLOGY,
FACULTY OF LIFE SCIENCES,
AHMADU BELLO UNIVERSITY,
ZARIA, KADUNA STATE